Double-stranded RNA and isometric virus-like particles in the entomopathogenic fungus Metarhizium anisopliae

被引：19

作者：

Bogo, MR ^{[1
]}

Queiroz, MV ^{[1
]}

Silva, DM ^{[1
]}

Gimenez, MP ^{[1
]}

Azevedo, JL ^{[1
]}

Schrank, A ^{[1
]}

机构：

[1] UNIV SAO PAULO,ESALQ,FAC AGR,BR-13418900 PIRACICABA,SP,BRAZIL

来源：

MYCOLOGICAL RESEARCH | 1996年 / 100卷

基金：

巴西圣保罗研究基金会;

关键词：

D O I：

10.1016/S0953-7562(96)80079-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Seven wild-type isolates of the entomopathogenic Metashizium anisopliae were analysed for the presence of a mycoviral infection. Extra bands of dsRNA were detected in three of them via agarose gel electrophoresis. Strain pi has bands of dsRNA varying from 910 to 3100 bp. One associated protein (46 kDa) was identified. The presence of virus-like particles of ca 35 nm of diameter was confirmed by TEM. The pattern of dsRNA was verified in both mycelium and spores. Auxotrophic mutants derived from strain RJ exhibited the same dsRNA band pattern and similar virus-like particles.

引用

页码：1468 / 1472

页数：5