Structural study of fucoidan from Cladosiphon okamuranus TOKIDA

被引：137

作者：

Nagaoka, M ^{[1
]}

Shibata, H ^{[1
]}

Kimura-Takagi, I ^{[1
]}

Hashimoto, S ^{[1
]}

Kimura, K ^{[1
]}

Makino, T ^{[1
]}

Aiyama, R ^{[1
]}

Ueyama, S ^{[1
]}

Yokokura, T ^{[1
]}

机构：

[1] Yakult Cent Inst Microbiol Res, Kunitachi, Tokyo 1868650, Japan

来源：

GLYCOCONJUGATE JOURNAL | 1999年 / 16卷 / 01期

关键词：

fucoidan; sulfated fucan; seaweed;

D O I：

10.1023/A:1006945618657

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A structural study was carried out on a fucoidan isolated from the brown seaweed Cladosiphon okamuranus. The polysaccharide contained fucose, glucuronic acid and sulfate in a molar ratio of about 6.1 : 1.0 : 2.9. The results of Smith degradation showed that this polysaccharide has a linear backbone of 1-->3-linked alpha-fucopyranose with a half sulfate substitution at the 4-positions, and a portion of the fucose residues was O-acetylated. The data obtained from partial acid hydrolysis, a methylation analysis and NMR spectra indicated that the alpha-glucuronic acid residue is linked to the 2-positions of the fucose residues, which were not substituted by a sulfate group. These results indicated that the average structure of this fucoidan is as follows: -[(-->3Fuc-4(+/-OSO3-)alpha 1-)(5)-->3[GlcA alpha 1-->2]Fuc alpha 1-](n)(-). (Half of each fucose residue was sulfated. One O-acetyl ester was present in every 6 fucose residues.).

引用

页码：19 / 26

页数：8

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