Structural study of fucoidan from Cladosiphon okamuranus TOKIDA

被引：137

作者：

Nagaoka, M ^{[1
]}

Shibata, H ^{[1
]}

Kimura-Takagi, I ^{[1
]}

Hashimoto, S ^{[1
]}

Kimura, K ^{[1
]}

Makino, T ^{[1
]}

Aiyama, R ^{[1
]}

Ueyama, S ^{[1
]}

Yokokura, T ^{[1
]}

机构：

[1] Yakult Cent Inst Microbiol Res, Kunitachi, Tokyo 1868650, Japan

来源：

GLYCOCONJUGATE JOURNAL | 1999年 / 16卷 / 01期

关键词：

fucoidan; sulfated fucan; seaweed;

D O I：

10.1023/A:1006945618657

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A structural study was carried out on a fucoidan isolated from the brown seaweed Cladosiphon okamuranus. The polysaccharide contained fucose, glucuronic acid and sulfate in a molar ratio of about 6.1 : 1.0 : 2.9. The results of Smith degradation showed that this polysaccharide has a linear backbone of 1-->3-linked alpha-fucopyranose with a half sulfate substitution at the 4-positions, and a portion of the fucose residues was O-acetylated. The data obtained from partial acid hydrolysis, a methylation analysis and NMR spectra indicated that the alpha-glucuronic acid residue is linked to the 2-positions of the fucose residues, which were not substituted by a sulfate group. These results indicated that the average structure of this fucoidan is as follows: -[(-->3Fuc-4(+/-OSO3-)alpha 1-)(5)-->3[GlcA alpha 1-->2]Fuc alpha 1-](n)(-). (Half of each fucose residue was sulfated. One O-acetyl ester was present in every 6 fucose residues.).

引用

页码：19 / 26

页数：8

共 42 条

[21] HIGHLY ACIDIC GLYCANS FROM SEA-CUCUMBERS - ISOLATION AND FRACTIONATION OF FUCOSE-RICH SULFATED POLYSACCHARIDES FROM THE BODY WALL OF LUDWIGOTHUREA-GRISEA [J].

MOURAO, PAS ;

BASTOS, IG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 166 (03) :639-645

[22]

MULLOY B, 1994, J BIOL CHEM, V269, P22113

[23] STRUCTURE OF POLYSACCHARIDE-PEPTIDOGLYCAN COMPLEX FROM THE CELL-WALL OF LACTOBACILLUS-CASEI YIT9018 [J].

NAGAOKA, M ;

MUTO, M ;

NOMOTO, K ;

MATUZAKI, T ;

WATANABE, T ;

YOKOKURA, T .

JOURNAL OF BIOCHEMISTRY, 1990, 108 (04) :568-571

[24] Structure of a galactan from cell walls of Bifidobacterium catenulatum YIT4016 [J].

Nagaoka, M ;

Hashimoto, S ;

Shibata, H ;

Kimura, I ;

Kimura, K ;

Sawada, H ;

Yokokura, T .

CARBOHYDRATE RESEARCH, 1996, 281 (02) :285-291

[25] STRUCTURAL STUDIES ON A CELL-WALL POLYSACCHARIDE FROM BIFIDOBACTERIUM-LONGUM YIT4028 [J].

NAGAOKA, M ;

SHIBATA, H ;

KIMURA, I ;

HASHIMOTO, S ;

KIMURA, K ;

SAWADA, H ;

YOKOKURA, T .

CARBOHYDRATE RESEARCH, 1995, 274 :245-249

[26] AN ANTICOAGULANT FUCOIDAN FROM THE BROWN SEAWEED ECKLONIA-KUROME [J].

NISHINO, T ;

KIYOHARA, H ;

YAMADA, H ;

NAGUMO, T .

PHYTOCHEMISTRY, 1991, 30 (02) :535-539

[27] ANTICOAGULANT AND ANTITHROMBIN ACTIVITIES OF OVERSULFATED FUCANS [J].

NISHINO, T ;

NAGUMO, T .

CARBOHYDRATE RESEARCH, 1992, 229 (02) :355-362

[28] STUDIES ON POLYSACCHARIDES FROM ECKLONIA-KUROME .2. STRUCTURAL CHARACTERIZATION OF A NEW ANTICOAGULANT FUCAN SULFATE FROM THE BROWN SEAWEED ECKLONIA-KUROME [J].

NISHINO, T ;

NAGUMO, T ;

KIYOHARA, H ;

YAMADA, H .

CARBOHYDRATE RESEARCH, 1991, 211 (01) :77-90

[29] THE INFLUENCE OF SULFATE CONTENT AND MOLECULAR-WEIGHT OF A FUCAN SULFATE FROM THE BROWN SEAWEED ECKLONIA-KUROME ON ITS ANTITHROMBIN ACTIVITY [J].

NISHINO, T ;

AIZU, Y ;

NAGUMO, T .

THROMBOSIS RESEARCH, 1991, 64 (06) :723-731

[30]

OEHNINGER S, 1991, FERTIL STERIL, V55, P165

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