Characterization and identification of vaccine candidate proteins through analysis of the group A Streptococcus surface proteome

被引：336

作者：

Rodríguez-Ortega, MJ ^{[1
]}

Norais, N ^{[1
]}

Bensi, G ^{[1
]}

Liberatori, S ^{[1
]}

Capo, S ^{[1
]}

Mora, M ^{[1
]}

Scarselli, M ^{[1
]}

Doro, F ^{[1
]}

Ferrari, G ^{[1
]}

Garaguso, I ^{[1
]}

Maggi, T ^{[1
]}

Neumann, A ^{[1
]}

Covre, A ^{[1
]}

Telford, JL ^{[1
]}

Grandi, G ^{[1
]}

机构：

[1] Chiron Vaccines, I-53100 Siena, Italy

来源：

NATURE BIOTECHNOLOGY | 2006年 / 24卷 / 02期

关键词：

D O I：

10.1038/nbt1179

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a proteomic approach for identifying bacterial surface-exposed proteins quickly and reliably for their use as vaccine candidates. Whole cells are treated with proteases to selectively digest protruding proteins that are subsequently identified by mass spectrometry analysis of the released peptides. When applied to the sequenced M1_SF370 group A Streptococcus strain, 68 PSORT-predicted surface-associated proteins were identified, including most of the protective antigens described in the literature. The number of surface-exposed proteins varied from strain to strain, most likely as a consequence of different capsule content. The surface-exposed proteins of the highly virulent M23_DSM2071 strain included 17 proteins, 15 in common with M1_SF370. When 14 of the 17 proteins were expressed in E. coli and tested in the mouse for their capacity to confer protection against a lethal dose of M23_DSM2071, one new protective antigen (Spy0416) was identified. This strategy overcomes the difficulties so far encountered in surface protein characterization and has great potential in vaccine discovery.

引用

页码：191 / 197

页数：7

共 52 条

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