Effects of pH, salt and time on ligand binding properties of overexpressed melanocortin 4 receptor

被引：2

作者：

Dolby, V

Lundqvist, A ^{[1
]}

Fröberg, T

Lüllau, E

Shaw, J

Tjerneld, F

Cronet, P

机构：

[1] AstraZeneca R&D, S-43183 Molndal, Sweden

[2] Lund Univ, Ctr Chem & Chem Engn, Dept Biochem, S-22100 Lund, Sweden

[3] AstraZeneca R&D, AstraZeneca Biotech Lab, S-15185 Sodertalje, Sweden

[4] AstraZeneca R&D, Macclesfield SK10 4TG, Cheshire, England

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2004年 / 58卷 / 03期

关键词：

melanocortin receptor; G-protein coupled receptor; ligand binding; MTII; NDP-MSH; antibodies;

D O I：

10.1016/j.jbbm.2003.10.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The G-protein coupled melanocortin 4 receptor (MC4r) plays an important role in the energy metabolism. We overexpressed the MC4r in CHO cells and performed characterisation studies on the cell membranes to determine functional stability and ligand binding properties of the receptor. The affinity for the ligands [Nle(4), D-Phe(7)]-alphaMSH and MTII was lost below pH 6 but could be restored by returning to physiological pH. Increasing NaCl concentration up to 1 M had little influence on the binding of either ligand. At neutral pH, physiological salt concentration and 4 degreesC the ligand affinity of the receptor was stable for up to 6 days. These findings will facilitate design of purification methods for the receptor. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：195 / 205

页数：11

共 24 条

[21] Selectivity of cyclic [D-Nal(7)] and [D-Phe(7)] substituted MSH analogues for the melanocortin receptor subtypes [J].