Eucalypt alpha-tubulin: CDNA cloning and increased level of transcripts in ectomycorrhizal root system

被引：23

作者：

Diaz, EC ^{[1
]}

Martin, F ^{[1
]}

Tagu, D ^{[1
]}

机构：

[1] INRA,CTR RECH NANCY,EQUIPE MICROBIOL FORESTIERE,F-54280 CHAMPENOUX,FRANCE

来源：

PLANT MOLECULAR BIOLOGY | 1996年 / 31卷 / 04期

关键词：

cytoskeleton; Eucalyptus globulus; gene expression; mycorrhiza; Pisolithus tinctorius; tubulin;

D O I：

10.1007/BF00019477

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (No. 270) of the ectomycorrhizal basidiomycete Pisolithus tinctorius caused no changes in root transcript levels of EgTubA1, whereas a drastic up-regulation in its expression was observed between 3 to 4 days after contact with the aggressive isolate 441. This enhanced alpha-tubulin expression coincided with the increase lateral root formation induced by fungal colonisation. The changes in alpha-tubulin expression support a role for cytoskeleton components in ectomycorrhiza development.

引用

页码：905 / 910

页数：6