ATP- and ADP-DnaA protein, a molecular switch in gene regulation

被引:158
作者
Speck, C [1 ]
Weigel, C [1 ]
Messer, W [1 ]
机构
[1] Max Planck Inst Mol Genet, D-14195 Berlin, Germany
关键词
autoregulation of dnaA; BIAcore; initiation of replication; oriC; repression;
D O I
10.1093/emboj/18.21.6169
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
DnaA protein functions by binding to asymmetric 9mer DNA sites, the DnaA boxes. ATP-DnaA and ADP-DnaA bind to 9mer DnaA boxes with equal affinity, but only ATP-DnaA protein binds in addition to an as yet unknown 6mer site, the ATP-DnaA box AGATCT, or a close match to it, ATP-DnaA protein binding to ATP-DnaA boxes is restricted to sites located in close proximity to DnaA boxes, suggesting that protein-protein interaction is required for its stabilization. We show that ATP-DnaA represses dnaA transcription much more efficiently than ADP-DnaA. DnaA is thus a regulatory molecule that, depending on the adenosine nucleotide bound, can bind to different sequences and thereby fulfill distinct functions.
引用
收藏
页码:6169 / 6176
页数:8
相关论文
共 42 条
  • [31] Sambrook J., 2002, MOL CLONING LAB MANU
  • [32] INTERACTION OF THE INITIATOR PROTEIN DNAA OF ESCHERICHIA-COLI WITH ITS DNA TARGET
    SCHAPER, S
    MESSER, W
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (29) : 17622 - 17626
  • [33] ATP ACTIVATES DNAA PROTEIN IN INITIATING REPLICATION OF PLASMIDS BEARING THE ORIGIN OF THE ESCHERICHIA-COLI CHROMOSOME
    SEKIMIZU, K
    BRAMHILL, D
    KORNBERG, A
    [J]. CELL, 1987, 50 (02) : 259 - 265
  • [34] SEKIMIZU K, 1988, J BIOL CHEM, V263, P7124
  • [35] THE INITIATOR PROTEIN DNAA - EVOLUTION, PROPERTIES AND FUNCTION
    SKARSTAD, K
    BOYE, E
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1994, 1217 (02): : 111 - 130
  • [36] Autoregulation of the Escherichia coli replication initiator protein, DnaA, is indirect
    Smith, RWP
    McAteer, S
    Masters, M
    [J]. MOLECULAR MICROBIOLOGY, 1997, 23 (06) : 1303 - 1315
  • [37] G protein mechanisms: Insights from structural analysis
    Sprang, SR
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1997, 66 : 639 - 678
  • [38] Interaction of the Escherichia coli DnaA protein with bacteriophage λ DNA
    Szalewska-Palasz, A
    Weigel, C
    Speck, C
    Srutkowska, S
    Konopa, G
    Lurz, R
    Marszalek, J
    Taylor, K
    Messer, W
    Wegrzyn, G
    [J]. MOLECULAR AND GENERAL GENETICS, 1998, 259 (06): : 679 - 688
  • [39] TRANSCRIPTIONAL REPRESSION OF THE DNAA GENE OF ESCHERICHIA-COLI BY DNAA PROTEIN
    WANG, QP
    KAGUNI, JM
    [J]. MOLECULAR & GENERAL GENETICS, 1987, 209 (03): : 518 - 525
  • [40] An ATP-ADP switch in MuB controls progression of the Mu transposition pathway
    Yamauchi, M
    Baker, TA
    [J]. EMBO JOURNAL, 1998, 17 (18) : 5509 - 5518