Construction of RNA nanocages by re-engineering the packaging RNA of Phi29 bacteriophage

被引：61

作者：

Hao, Chenhui ^{[1
]}

Li, Xiang ^{[1
]}

Tian, Cheng ^{[1
]}

Jiang, Wen ^{[2
]}

Wang, Guansong ^{[3
]}

Mao, Chengde ^{[1
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

[2] Purdue Univ, Dept Biol Sci, Markey Ctr Struct Biol, W Lafayette, IN 47907 USA

[3] Xinqiao Hosp, Inst Resp Dis, Chongqing 400037, Peoples R China

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

关键词：

3-WAY JUNCTION; IN-VITRO; DNA; NANOPARTICLES; COMPLEX; NANOTECHNOLOGY; FABRICATION; PATTERNS; DELIVERY; SHAPES;

D O I：

10.1038/ncomms4890

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

RNA nanotechnology promises rational design of RNA nanostructures with wide array of structural diversities and functionalities. Such nanostructures could be used in applications such as small interfering RNA delivery and organization of in vivo chemical reactions. Though having impressive development in recent years, RNA nanotechnology is still quite limited and its programmability and complexity could not rival the degree of its closely related cousin: DNA nanotechnology. Novel strategies are needed for programmed RNA self-assembly. Here, we have assembled RNA nanocages by re-engineering a natural, biological RNA motif: the packaging RNA of phi29 bacteriophage. The resulting RNA nanostructures have been thoroughly characterized by gel electrophoresis, cryogenic electron microscopy imaging and dynamic light scattering.

引用

页数：7

共 38 条

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