共 40 条
Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation
被引:306
作者:

Steffen, A
论文数: 0 引用数: 0
h-index: 0
机构: German Res Ctr Biotechnol, Dept Cell Biol, D-38124 Braunschweig, Germany

Rottner, K
论文数: 0 引用数: 0
h-index: 0
机构: German Res Ctr Biotechnol, Dept Cell Biol, D-38124 Braunschweig, Germany

Ehinger, J
论文数: 0 引用数: 0
h-index: 0
机构: German Res Ctr Biotechnol, Dept Cell Biol, D-38124 Braunschweig, Germany

论文数: 引用数:
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论文数: 引用数:
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Wehland, J
论文数: 0 引用数: 0
h-index: 0
机构: German Res Ctr Biotechnol, Dept Cell Biol, D-38124 Braunschweig, Germany

Stradal, TEB
论文数: 0 引用数: 0
h-index: 0
机构: German Res Ctr Biotechnol, Dept Cell Biol, D-38124 Braunschweig, Germany
机构:
[1] German Res Ctr Biotechnol, Dept Cell Biol, D-38124 Braunschweig, Germany
[2] European Inst Oncol, Dept Expt Oncol, Milan, Italy
关键词:
Abi;
lamellipodium;
Rac;
WAVE;
D O I:
10.1038/sj.emboj.7600084
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The Rho-GTPase Rac1 stimulates actin remodelling at the cell periphery by relaying signals to Scar/WAVE proteins leading to activation of Arp2/3-mediated actin polymerization. Scar/WAVE proteins do not interact with Rac1 directly, but instead assemble into multiprotein complexes, which was shown to regulate their activity in vitro. However, little information is available on how these complexes function in vivo. Here we show that the specifically Rac1-associated protein-1 (Sra-1) and Nck-associated protein 1 (Nap1) interact with WAVE2 and Abi-1 (e3B1) in resting cells or upon Rac activation. Consistently, Sra-1, Nap1, WAVE2 and Abi-1 translocated to the tips of membrane protrusions after microinjection of constitutively active Rac. Moreover, removal of Sra-1 or Nap1 by RNA interference abrogated the formation of Rac-dependent lamellipodia induced by growth factor stimulation or aluminium fluoride treatment. Finally, microinjection of an activated Rac failed to restore lamellipodia protrusion in cells lacking either protein. Thus, Sra-1 and Nap1 are constitutive and essential components of a WAVE2- and Abi-1-containing complex linking Rac to site-directed actin assembly.
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页码:749 / 759
页数:11
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