Structure of a RSC-nucleosome complex and insights into chromatin remodeling

被引：104

作者：

Chaban, Yuriy ^{[1
]}

Ezeokonkwo, Chukwudi ^{[1
]}

Chung, Wen-Hsiang ^{[1
]}

Zhang, Fan ^{[1
]}

Kornberg, Roger D. ^{[2
]}

Maier-Davis, Barbara ^{[2
]}

Lorch, Yahli ^{[2
]}

Asturias, Francisco J. ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[2] Stanford Univ, Dept Biol Struct, Med Ctr, Stanford, CA 94035 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2008年 / 15卷 / 12期

关键词：

D O I：

10.1038/nsmb.1524

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ATP-dependent chromatin-remodeling complexes, such as RSC, can reposition, evict or restructure nucleosomes. A structure of a RSC -nucleosome complex with a nucleosome determined by cryo-EM shows the nucleosome bound in a central RSC cavity. Extensive interaction of RSC with histones and DNA seems to destabilize the nucleosome and lead to an overall ATP-independent rearrangement of its structure. Nucleosomal DNA appears disordered and largely free to bulge out into solution as required for remodeling, but the structure of the RSC -nucleosome complex indicates that RSC is unlikely to displace the octamer from the nucleosome to which it is bound. Consideration of the RSC -nucleosome structure and published biochemical information suggests that ATP-dependent DNA translocation by RSC may result in the eviction of histone octamers from adjacent nucleosomes.

引用

页码：1272 / 1277

页数：6

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