Use of reverse transcription polymerase chain reaction with colorimetric plate hybridization to detect a cytomegalovirus late spliced mRNA in polymorphonuclear leukocytes from renal transplant patients

Human cytomegalovirus replication was evaluated in polymorphonuclear leukocytes from ten renal transplant recipients. Three new reverse transcription polymerase chain reactions with plate hybridization suitable for automation were developed for the detection of immediate-early spliced U(L)123 mRNA, early-late pp65 mRNA, and late spliced U(L)22 mRNA. The presence of U(L)22mRNA was found to be significantly associated with the occurrence of cytomegalovirus (CMV) disease. (C) 1999 Elsevier Science Inc.