COLORIMETRIC MICROTITER PLATE HYBRIDIZATION ASSAY USING MONOCLONAL-ANTIBODY FOR DETECTION OF AN AMPLIFIED HUMAN-IMMUNODEFICIENCY-VIRUS TARGET

被引：10

作者：

FERREAUBINEAU, V ^{[1
]}

IMBERTMARCILLE, BM ^{[1
]}

RAFFI, F ^{[1
]}

BESSE, B ^{[1
]}

LOIRAT, R ^{[1
]}

BILLAUDEL, S ^{[1
]}

机构：

[1] NANTES UNIV HOSP,DEPT INTERNAL MED,NANTES,FRANCE

来源：

JOURNAL OF VIROLOGICAL METHODS | 1995年 / 55卷 / 01期

关键词：

HUMAN IMMUNODEFICIENCY VIRUS; COLORIMETRIC MICROTITER PLATE HYBRIDIZATION ASSAY; DNA ENZYME IMMUNOASSAY; MONOCLONAL ANTIBODY;

D O I：

10.1016/0166-0934(95)00058-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Since detection of amplified polymerase chain reaction products is a complicated procedure, a colorimetric microtiter plate hybridization assay was developed to automate specific detection of amplified HIV targets. In this assay, hybridization is seen by an antibody reacting selectively with double-stranded DNA. One hundred and ten amplified products detected by a DNA enzyme immunoassay (DEIA) and by classical hybridization with a digoxigenin-labeling probe, detection sensitivities were less than 10 HIV targets per 10(6) cells. This study demonstrates the specificity and sensitivity of DEIA for detecting amplified HIV targets. The use of the same equipment as for ELISA and the complete automation of the procedure allow a large number of samples to be processed in the clinical laboratory.

引用

页码：145 / 151

页数：7

共 16 条

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