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Should gaps be included in chromosomal aberration analysis?: Evidence based on the comet assay

被引：37
作者：
Paz-y-Miño, C
Dávalos, MV
Sánchez, ME
Arévalo, M
Leone, PE
机构：
[1] Fac Ciencias Exactas & Nat, Dept Ciencias Biol, Lab Genet Mol & Citogenet Humana, Quito, Ecuador
[2] Pontificia Univ Catolica Ecuador, Fac Med, Area Genet, Quito, Ecuador
来源：
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2002年 / 516卷 / 1-2期
关键词：
comet assay; chromosomal aberration; gap;
D O I：
10.1016/S1383-5718(02)00021-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
This study evaluated DNA damage in human lymphocytes due to occupational exposure to low levels of ionizing radiation using two assays: the comet assay and chromosomal aberration (CA) analysis including and excluding gaps. The results obtained reveal a higher correlation between both methods when chromatid and chromosome gaps were included in the correlation analysis (r = 0.78 versus r = 0.50). This increased correlation support the hypothesis that the gaps constitute a type of chromosome aberration, and suggest that these events should be scored in this type of analysis. (C) 2002 Published by Elsevier Science B.V.
引用
收藏
页码：57 / 61
页数：5
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