Screen anticancer drug in vitro using resonance light scattering technique

被引：32

作者：

Chen, Zhanguang ^{[1
]}

Liu, Guoliang ^{[1
,2
]}

Chen, Meizhen ^{[2
]}

Xu, Benjie ^{[2
]}

Peng, Yurui ^{[1
]}

Chen, Maohuai ^{[3
]}

Wu, Mingyao ^{[3
]}

机构：

[1] Shantou Univ, Dept Chem, Shantou 515063, Peoples R China

[2] Shantou Univ, Dept Biol, Shantou 515063, Peoples R China

[3] Shantou Univ, Coll Med, Shantou 515041, Peoples R China

来源：

TALANTA | 2009年 / 77卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Resonance light scattering; Screen in vitro; Anticancer drug; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; Hepatocellular carcinoma cell of mouse; RAYLEIGH-SCATTERING; NUCLEIC-ACIDS; WATER/TETRACHLOROMETHANE INTERFACE; CETYLTRIMETHYLAMMONIUM BROMIDE; HEPATOCELLULAR-CARCINOMA; SILVER NANOPARTICLES; CELL-CULTURE; LUNG-CANCER; ASSAY; COMPLEX;

D O I：

10.1016/j.talanta.2008.09.016

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An in vitro screening model using resonance light scattering (RLS) technique with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reagent as the reactive probe to target cancer cell was firstly developed. In this model, MTT was reduced by viable cancer cells to produce a purple formazan. Cell viability was proportional to the number of formazan induced strong light scattering signal. The inhibition rate of anticancer drug was found to vary inversely with the H-22-MTT system RLS intensity. So it was intuitive to see the sequence of the tumor suppressive activity of six anticancer drugs without data processing by RLS/MTT screening spectra. Compared with the traditional MTT method, this method has high sensitivity, low detection limit and quite intuitive screening results which were identical to those obtained from the MTT colorimetric assay. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1365 / 1369

页数：5

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