The effect of heat pretreatment temperature on fermentative hydrogen production using mixed cultures

被引：139

作者：

Baghchehsaraee, Bita ^{[1
]}

Nakhla, George ^{[1
]}

Karamanev, Dimitre ^{[1
]}

Margaritis, Argyrios ^{[1
]}

Reid, Gregor ^{[2
,3
]}

机构：

[1] Univ Western Ontario, Dept Chem & Biochem Engn, London, ON N6A 5B9, Canada

[2] Univ Western Ontario, Dept Microbiol & Immunol, London, ON N6A 5B9, Canada

[3] Lawson Hlth Res Inst, Canadian Res & Dev Ctr Probiot, London, ON N6A 4V2, Canada

来源：

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY | 2008年 / 33卷 / 15期

基金：

加拿大自然科学与工程研究理事会;

关键词：

biological hydrogen production; heat treatment; inoculum enrichment; PCR-DGGE; butyrate/acetate ratio; Clostridium;

D O I：

10.1016/j.ijhydene.2008.05.069

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

sludge and anaerobically digested sludge, was investigated in batch cultures. Heat treatments were conducted at 65, 80 and 95 degrees C for 30 min. The untreated inocula produced less amount of hydrogen than the pretreated inocula, with lactic acid as the main metabolite. The maximum yields of 2.3 and 1.6 mol H-2/mol glucose were achieved for the 65 degrees C pretreated anaerobically digested and activated sludges, respectively. Approximately a 15% decrease in yield was observed with increasing pretreatment temperature from 65 to 95 degrees C concomitant with an increase in butyrate/acetate ratio from 1.5 to 2.4 for anaerobically digested sludge. The increase of pretreatment temperature of activated sludge to 95 degrees C suppressed the hydrogen production by lactic acid fermentation. DNA analysis of the microbial community showed that the elevated pretreatment temperatures reduced the species diversity. (C) 2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.

引用

页码：4064 / 4073

页数：10