Use of an autosampler for dynamic headspace extraction of volatile compounds from grains and effect of added water on the extraction

被引:12
作者
Ram, MS
Seitz, LM
Rengarajan, R
机构
[1] ARS, US Grain Mkt Res Lab, Grain Mkt & Prod Res Ctr, Manhattan, KS 66502 USA
[2] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA
关键词
grain volatiles; headspace autosampler; odor; soybean; 1-octen-3-ol;
D O I
10.1021/jf990243e
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
An autosampler attached to a purge and trap instrument was used to aid routine analyses of grain samples for volatile compounds associated with off-odors. Trapped volatiles were transferred to a gas chromatograph/mass spectrometer instrument for separation and detection. Dynamic extraction of volatiles from similar to 18 g of whole grain at 80 degrees C was accomplished by purging helium through a sample vial with a Teflon-lined septum on each end. The autosampler automatically added internal standard to the sample before purging began, which required the addition of 1 mL of water for complete transfer of the standard to the sample. The added water enhanced extraction of 1-octen-3-ol, 1-octen-3-one, and some other compounds from soybeans but-not from starchy grains such as corn and wheat. Addition of a free radical scavenger, such as citric acid, greatly diminished the recovery of 1-octen-3-ol and 1-octen-3-one from soybeans.
引用
收藏
页码:4202 / 4208
页数:7
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