Fast imaging and fast force spectroscopy of single biopolymers with a new atomic force microscope designed for small cantilevers

被引:207
作者
Viani, MB [1 ]
Schäffer, TE
Paloczi, GT
Pietrasanta, LI
Smith, BL
Thompson, JB
Richter, M
Rief, M
Gaub, HE
Plaxco, KW
Cleland, AN
Hansma, HG
Hansma, PK
机构
[1] Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA
[2] Univ Munich, Lehrstuhl Angew Phys, D-80799 Munich, Germany
[3] Univ Calif Santa Barbara, Dept Chem, Santa Barbara, CA 93106 USA
关键词
D O I
10.1063/1.1150069
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
Small cantilevers allow for faster imaging and faster force spectroscopy of single biopolymers than previously possible because they have higher resonant frequencies and lower coefficients of viscous damping. We have used a new prototype atomic force microscope with small cantilevers to produce stable tapping-mode images (1 mu mx1 mu m) in liquid of DNA adsorbed onto mica in as little as 1.7 s per image. We have also used these cantilevers to observe the forced unfolding of individual titin molecules on a time scale an order of magnitude faster than previously reported. These experiments demonstrate that a new generation of atomic force microscopes using small cantilevers will enable us to study biological processes with greater time resolution. Furthermore, these instruments allow us to narrow the gap in time between results from force spectroscopy experiments and molecular dynamics calculations. (C) 1999 American Institute of Physics. [S0034-6748(99)02411-9].
引用
收藏
页码:4300 / 4303
页数:4
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