Osteopontin-deficient mice are resistant to ovariectomy-induced bone resorption

被引:292
作者
Yoshitake, H
Rittling, SR
Denhardt, DT
Noda, M
机构
[1] Tokyo Med & Dent Univ, Med Res Inst, Dept Mol Pharmacol, Chiyoda Ku, Tokyo, Japan
[2] Rutgers State Univ, Dept Cell Biol & Neurosci, Piscataway, NJ USA
关键词
D O I
10.1073/pnas.96.14.8156
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Osteopontin is one of the major noncollagenous bone matrix proteins produced by osteoblasts and osteoclasts, bone cells that are uniquely responsible for the remodeling of mineralized tissues, Osteoclasts express the alpha v beta 3 integrin, which is one of the receptors for osteopontin, Recent knockout studies revealed that noncollagenous bone matrix proteins are functionally important in regulation of bone metabolism, However, the significance of the presence of osteopontin in in vivo has not been known. We report here that osteopontin knockout mice are resistant to ovariectomy-induced bone resorption compared with wild-type mice. Microcomputed tomography analysis indicated about 60% reduction in bone volume by ovariectomy in wild-type,mice, whereas the osteopontin-deficient mice exhibited only about 10% reduction in trabecular bone volume after ovariectomy, Reduction in uterine weight was observed similarly in both wild-type and osteopontin-deficient mice, indicating the specificity of the effect of osteopontin deficiency on bone metabolism. We propose that osteopontin is essential for postmenopausal osteoporosis in women. Strategies to counteract osteopontin's action may prove effective in suppressing osteoporosis.
引用
收藏
页码:8156 / 8160
页数:5
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