Antigenotoxic properties of Eruca sativa (rocket plant), erucin and erysolin in human hepatoma (HepG2) cells towards benzo(a)pyrene and their mode of action

被引：56

作者：

Lamy, Evelyn ^{[1
]}

Schroeder, Julia ^{[1
]}

Paulus, Stefanie ^{[1
]}

Brenk, Peter ^{[1
]}

Stahl, Thorsten ^{[2
]}

Mersch-Sundermann, Volker ^{[1
]}

机构：

[1] Univ Med Ctr Freiburg, Inst Environm Med & Hosp Hyg, D-79106 Freiburg, Germany

[2] Abt Umwelt & Spurenanalyt 5, Landesbetrieb Hess Landeslabor, D-65203 Wiesbaden, Germany

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2008年 / 46卷 / 07期

关键词：

Brassicaceae; Eruca sativa; rocket plant; comet assay; HepG2; cells; antigenotoxicity;

D O I：

10.1016/j.fct.2008.03.022

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In recent years, rocket plant (Eruca sativa) has gained greater importance as a vegetable and spice, especially among Europeans. E. sativa is a member of the Brassicaceae, which is considered to be an important chemopreventive plant family. In the present study, we assessed the chemopreventive potency and underlying mechanisms of extracts of E sativa in HepG2 cells. No genotoxic effect could be observed in HepG2 cells treated with up to 50 mu l/ml plant juice for 24 h when using the comet assay. In antigenotoxicity experiments, E sativa extract reduced the benzo(a)pyrene-induced genotoxicity in a U-shaped This effect was accompanied by a significant induction of glutathione S-transferase. No signifi-manner. cant suppression of B(a)P-induced CYP1A1 protein expression or enzyme activity could be observed. Chemical analysis of the plant material by gas chromatography identified the isothiocyanates erucin, sulforaphane, erysolin and phenylethyl isothiocyanate. Results derived with the single ITC compounds support the assumption that their synergistic interaction is responsible for the strong antigenotoxicity of the plant material. The present study provided an assessment of the bioactive effects of rocket plant extract in a human cell culture system. This could help to evaluate the balance between beneficial vs. possible adverse effects of rocket plant consumption. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2415 / 2421

页数：7

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