Comparison of methods and animal models commonly used for investigation of fecal microbiota: Effects of time, host and gender

被引：36

作者：

Bernbom, Nete

Norrung, Birgit

Saadbye, Peter

Molbak, Lars

Vogensen, Finn K.

Licht, Tine Rask

机构：

[1] Danish Inst Food & Vet Res, Dept Microbiol Food Safety, DK-2860 Soborg, Denmark

[2] Danish Inst Food & Vet Res, Dept Vet Diagnost & Res, DK-1790 Copenhagen, Denmark

[3] Royal Vet & Agr Univ, Dept Food Sci, DK-1870 Frederiksberg, Denmark

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 66卷 / 01期

关键词：

DGGE; T-RFLP; fecal microbiota; community analysis;

D O I：

10.1016/j.mimet.2005.10.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP) and plating on selective agars were used to study variation in the fecal microbiota of rats over time as well as variation between individuals. Investigated rats were either conventional and specific pathogen free (SPF). or human flora associated (HFA). A higher variation (p < 0.05) in fecal microbiota over time was observed for HFA than for SPF animals. Analysis of DGGE and T-RFLP profiles of fecal microbiota from SPF and HFA rats revealed that variation over time was less significant than variation between individuals, and that phylogenetic profiles clustered according to gender. These observations should be taken into account when designing future research addressing changes in fecal microbiota. (c) 2005 Elsevier B.V. All rights reserved.

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收藏

页码：87 / 95

页数：9

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