Syndecan-syntenin-ALIX regulates the biogenesis of exosomes

被引：1397

作者：

Baietti, Maria Francesca ^{[1
,2
,3
]}

Zhang, Zhe ^{[2
,3
]}

Mortier, Eva ^{[1
]}

Melchior, Aurelie ^{[2
,3
]}

Degeest, Gisele ^{[2
,3
]}

Geeraerts, Annelies ^{[1
,4
]}

Ivarsson, Ylva ^{[1
]}

Depoortere, Fabienne ^{[1
]}

Coomans, Christien ^{[2
,3
]}

Vermeiren, Elke ^{[1
]}

Zimmermann, Pascale ^{[1
]}

David, Guido ^{[2
,3
]}

机构：

[1] KULeuven, Dept Human Genet, Lab Signal Integrat Cell Fate Decis, B-3000 Louvain, Belgium

[2] KULeuven, Dept Human Genet, Lab Glycobiol & Dev Genet, B-3000 Louvain, Belgium

[3] VIB, Ctr Biol Dis, B-3000 Louvain, Belgium

[4] KULeuven, Dept Chem, Lab Biomol Dynam, B-3001 Heverlee, Belgium

来源：

NATURE CELL BIOLOGY | 2012年 / 14卷 / 07期

关键词：

MULTIVESICULAR BODY BIOGENESIS; HEPARAN-SULFATE PROTEOGLYCANS; ESCRT MACHINERY; PLASMA-MEMBRANE; DOMAIN BINDING; CELL-ADHESION; PDZ PROTEIN; MECHANISM; ENDOSOMES; VESICLES;

D O I：

10.1038/ncb2502

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The biogenesis of exosomes, small secreted vesicles involved in signalling processes, remains incompletely understood. Here, we report evidence that the syndecan heparan sulphate proteoglycans and their cytoplasmic adaptor syntenin control the formation of exosomes. Syntenin interacts directly with ALIX through LYPX(n)L motifs, similarly to retroviral proteins, and supports the intraluminal budding of endosomal membranes. Syntenin exosomes depend on the availability of heparan sulphate, syndecans, ALIX and ESCRTs, and impact on the trafficking and confinement of FGF signals. This study identifies a key role for syndecan syntenin ALIX in membrane transport and signalling processes.

引用

页码：677 / 685

页数：9

共 55 条

[1] Intercellular transfer of the oncogenic receptor EGFrvIII by microvesicles derived from tumour cells [J].

Al-Nedawi, Khalid ;

Meehan, Brian ;

Micallef, Johann ;

Lhotak, Vladimir ;

May, Linda ;

Guha, Abhijit ;

Rak, Janusz .

NATURE CELL BIOLOGY, 2008, 10 (05) :619-U24

[2] MVB vesicle formation: ESCRT-dependent, ESCRT-independent and everything in between [J].

Babst, Markus .

CURRENT OPINION IN CELL BIOLOGY, 2011, 23 (04) :452-457

[3] The ins and outs of syntenin, a multifunctional intracellular adaptor protein [J].

Beekman, Jeffrey M. ;

Coffer, Paul J. .

JOURNAL OF CELL SCIENCE, 2008, 121 (09) :1349-1355

[4] Heparan sulphate proteoglycans fine-tune mammalian physiology [J].

Bishop, Joseph R. ;

Schuksz, Manuela ;

Esko, Jeffrey D. .

NATURE, 2007, 446 (7139) :1030-1037

[5] ATPase-defective mammalian VPS4 localizes to aberrant endosomes and impairs cholesterol trafficking [J].

Bishop, N ;

Woodmane, P .

MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (01) :227-239

[6] Exosome Secretion: Molecular Mechanisms and Roles in Immune Responses [J].

Bobrie, Angelique ;

Colombo, Marina ;

Raposo, Graca ;

Thery, Clotilde .

TRAFFIC, 2011, 12 (12) :1659-1668

[7] Transmembrane Signaling Proteoglycans [J].

Couchman, John R. .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 26, 2010, 26 :89-114

[8] Cell biology - The ins and outs of exosomes [J].

Couzin, J .

SCIENCE, 2005, 308 (5730) :1862-1863

[9] Depletion of TSG101 forms a mammalian 'Class E' compartment: a multicisternal early endosome with multiple sorting defects [J].

Doyotte, A ;

Russell, MRG ;

Hopkins, CR ;

Woodman, PG .

JOURNAL OF CELL SCIENCE, 2005, 118 (14) :3003-3017

[10] Higher-order oligomerization targets plasma membrane proteins and HIV gag to exosomes [J].

Fang, Yi ;

Wu, Ning ;

Gan, Xin ;

Yan, Wanhua ;

Morrell, James C. ;

Gould, Stephen J. .

PLOS BIOLOGY, 2007, 5 (06) :1267-1283

← 1 2 3 4 5 6 →