Syndecan-syntenin-ALIX regulates the biogenesis of exosomes

被引：1397

作者：

Baietti, Maria Francesca ^{[1
,2
,3
]}

Zhang, Zhe ^{[2
,3
]}

Mortier, Eva ^{[1
]}

Melchior, Aurelie ^{[2
,3
]}

Degeest, Gisele ^{[2
,3
]}

Geeraerts, Annelies ^{[1
,4
]}

Ivarsson, Ylva ^{[1
]}

Depoortere, Fabienne ^{[1
]}

Coomans, Christien ^{[2
,3
]}

Vermeiren, Elke ^{[1
]}

Zimmermann, Pascale ^{[1
]}

David, Guido ^{[2
,3
]}

机构：

[1] KULeuven, Dept Human Genet, Lab Signal Integrat Cell Fate Decis, B-3000 Louvain, Belgium

[2] KULeuven, Dept Human Genet, Lab Glycobiol & Dev Genet, B-3000 Louvain, Belgium

[3] VIB, Ctr Biol Dis, B-3000 Louvain, Belgium

[4] KULeuven, Dept Chem, Lab Biomol Dynam, B-3001 Heverlee, Belgium

来源：

NATURE CELL BIOLOGY | 2012年 / 14卷 / 07期

关键词：

MULTIVESICULAR BODY BIOGENESIS; HEPARAN-SULFATE PROTEOGLYCANS; ESCRT MACHINERY; PLASMA-MEMBRANE; DOMAIN BINDING; CELL-ADHESION; PDZ PROTEIN; MECHANISM; ENDOSOMES; VESICLES;

D O I：

10.1038/ncb2502

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The biogenesis of exosomes, small secreted vesicles involved in signalling processes, remains incompletely understood. Here, we report evidence that the syndecan heparan sulphate proteoglycans and their cytoplasmic adaptor syntenin control the formation of exosomes. Syntenin interacts directly with ALIX through LYPX(n)L motifs, similarly to retroviral proteins, and supports the intraluminal budding of endosomal membranes. Syntenin exosomes depend on the availability of heparan sulphate, syndecans, ALIX and ESCRTs, and impact on the trafficking and confinement of FGF signals. This study identifies a key role for syndecan syntenin ALIX in membrane transport and signalling processes.

引用

页码：677 / 685

页数：9

共 55 条

[51] Structural and functional studies of ALIX interactions with YPXnL late domains of HIV-1 and EIAV [J].

Zhai, Qianting ;

Fisher, Robert D. ;

Chung, Hyo-Young ;

Myszka, David G. ;

Sundquist, Wesley I. ;

Hill, Christopher P. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2008, 15 (01) :43-49

[52] Decoding the intrinsic mechanism that prohibits ALIX interaction with ESCRT and viral proteins [J].

Zhou, Xi ;

Si, Jiali ;

Corvera, Joe ;

Gallick, Gary E. ;

Kuang, Jian .

BIOCHEMICAL JOURNAL, 2010, 432 :525-534

[53] Syndecan recyling is controlled by syntenin-PIP2 interaction and AM [J].

Zimmermann, P ;

Zhang, Z ;

Degeest, G ;

Mortier, E ;

Leenaerts, I ;

Coomans, C ;

Schulz, J ;

N'Kuli, F ;

Courtoy, PJ ;

David, G .

DEVELOPMENTAL CELL, 2005, 9 (03) :377-388

[54] PIP2-PDZ domain binding controls the association of syntenin with the plasma membrane [J].

Zimmermann, P ;

Meerschaert, K ;

Reekmans, G ;

Leenaerts, I ;

Small, JV ;

Vandekerckhove, J ;

David, G ;

Gettemans, J .

MOLECULAR CELL, 2002, 9 (06) :1215-1225

[55] Characterization of syntenin, a syndecan-binding PDZ protein, as a component of cell adhesion sites and microfilaments [J].

Zimmermann, P ;

Tomatis, D ;

Rosas, M ;

Grootjans, J ;

Leenaerts, I ;

Degeest, G ;

Reekmans, G ;

Coomans, C ;

David, G .

MOLECULAR BIOLOGY OF THE CELL, 2001, 12 (02) :339-350

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