Effective, homogeneous and transient interference with cytosine methylation in plant genomic DNA by zebularine

被引：104

作者：

Baubec, Tuncay ^{[1
]}

Pecinka, Ales ^{[1
]}

Rozhon, Wilfried ^{[1
]}

Mittelsten Scheid, Ortrun ^{[1
]}

机构：

[1] Austrian Acad Sci, Gregor Mendel Inst Mol Plant Biol, A-1030 Vienna, Austria

来源：

PLANT JOURNAL | 2009年 / 57卷 / 03期

基金：

奥地利科学基金会;

关键词：

DNA methylation; methylation inhibitor; zebularine; epigenetic regulation; transcriptional reactivation; Arabidopsis; RIBOSOMAL-RNA GENES; ARABIDOPSIS-THALIANA; FUNCTIONAL-ANALYSIS; IN-VITRO; 5-AZACYTIDINE; CANCER; METHYLTRANSFERASES; REACTIVATION; MAINTENANCE; INHIBITION;

D O I：

10.1111/j.1365-313X.2008.03699.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Covalent modification by methylation of cytosine residues represents an important epigenetic hallmark. While sequence analysis after bisulphite conversion allows correlative analyses with single-base resolution, functional analysis by interference with DNA methylation is less precise, due to the complexity of methylation enzymes and their targets. A cytidine analogue, 5-azacytidine, is frequently used as an inhibitor of DNA methyltransferases, but its rapid degradation in aqueous solution is problematic for culture periods of longer than a few hours. Application of zebularine, a more stable cytidine analogue with a similar mode of action that is successfully used as a methylation inhibitor in Neurospora and mammalian tumour cell lines, can significantly reduce DNA methylation in plants in a dose-dependent and transient manner independent of sequence context. Demethylation is connected with transcriptional reactivation and partial decondensation of heterochromatin. Zebularine represents a promising new and versatile tool for investigating the role of DNA methylation in plants with regard to transcriptional control, maintenance and formation of (hetero-) chromatin.

引用

页码：542 / 554

页数：13

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