A sporulation membrane protein tethers the pro-σK processing enzyme to its inhibitor and dictates its subcellular localization

被引:95
作者
Rudner, DZ [1 ]
Losick, R [1 ]
机构
[1] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA
关键词
regulated proteolysis; subcellular localization; sporulation; sigma factor; transcription;
D O I
10.1101/gad.977702
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The developmental transcription factor sigma(K) is derived from the inactive precursor protein pro-sigma(K) by regulated proteolysis during the process of sporulation in the bacterium Bacillus subtilis. The putative pro-sigma(K) processing enzyme SpoIVFB is a member of a family of membrane-embedded metalloproteases and is held inactive by two other integral membrane proteins, SpoIVFA and BofA. Herein we show that the processing enzyme and its two regulators exist in a multimeric complex that localizes to the membrane surrounding the developing spore (the forespore). We further show that one of the regulators, SpoIVFA, plays a central role in both the formation of this complex and its subcellular localization. Evidence is presented in support of a model in which SpoIVFA acts as a platform for bringing BofA and SpoIVFB together, whereby BofA inhibits pro-sigma(K) processing until a signal has been received from the forespore.
引用
收藏
页码:1007 / 1018
页数:12
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