Expression and hormone regulation of prolactin receptors in rat dorsal and lateral prostate

被引:80
作者
Nevalainen, MT
Valve, EM
Ingleton, PM
Harkonen, PL
机构
[1] TURKU UNIV, INST BIOMED, DEPT ANAT, FIN-20520 TURKU, FINLAND
[2] TURKU UNIV, MED RES LAB, FIN-20520 TURKU, FINLAND
[3] UNIV SHEFFIELD, SCH MED, INST CANC STUDIES, SHEFFIELD S10 2TN, S YORKSHIRE, ENGLAND
关键词
D O I
10.1210/en.137.7.3078
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We have studied the receptors that presumably mediate the biological effects of PRL in rat dorsal (DP) and lateral (LP) prostate. The PRL receptor proteins mere localized to the glandular secretory epithelium of prostatic tis sue by immunohistochemistry. Both the short and the long PRL receptor proteins were detected in DP and LP by Western blot analysis and cross-linking of [I-125]human PRL to membrane preparations of DP and LP. Three messenger RNAs (mRNAs) for the long [1.3-1.7, 2.5, and 9.5-10 kilobases (kb)] and short (0.6-0.7, 3.0-4.6, and 10-12 kb) PRL receptors were expressed in dorsal and lateral lobes of rat prostate. Testosterone (T), estrogen (E), and PRL regulation of PRL receptor expression in rat DP and LP was studied in organ culture, which has been shown to be a suitable model to study hormone responses of prostatic tissue in vitro. The mRNAs of the short and long PRL receptors were differentially regulated in rat dorsolateral prostate. T, E, and PRL regulated the level of the long PRL receptor mRNAs in a tissue-specific manner, whereas hormone regulation of the short PRL receptor mRNAs was only modest. Furthermore, the hormonal responses of the different mRNA splicing variants of the long PRL receptor were not all similar; T, E, and PRL each increased the expression of 1.3- to 1.7-kb and 9.5- to 10-kb transcripts in DP, but only T did so in LP, whereas no clear regulation for the 2.5-kb mRNA could be observed in either tissue. This suggests that the hormonal regulation occurs at least at the posttranscriptional level. The effects of T and E were counteracted by the antihormones cyproterone and toremifene, respectively, indicating a specific receptor-mediated manner of steroid action.
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页码:3078 / 3088
页数:11
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