STED microscopy reveals crystal colour centres with nanometric resolution

被引:611
作者
Rittweger, Eva [1 ]
Han, Kyu Young [1 ]
Irvine, Scott E. [1 ]
Eggeling, Christian [1 ]
Hell, Stefan W. [1 ]
机构
[1] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany
基金
加拿大自然科学与工程研究理事会;
关键词
SINGLE DEFECT CENTERS; FLUORESCENCE MICROSCOPY; OPTICAL MICROSCOPY; DIAMOND; DEPLETION; NANOSCOPY; SPIN; LIMIT;
D O I
10.1038/NPHOTON.2009.2
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Because they have spin states that can be optically polarized and detected, fluorescent nitrogen vacancies in diamond(1-3) have considerable potential for applications in quantum cryptography(4,5) and computation(6-8), as well as for nanoscale magnetic imaging(9,10) and biolabelling(11,12). However, their optical detection and control are hampered by the diffraction resolution barrier of far-field optics. Here, we show that stimulated emission depletion (STED) microscopy(13,14) is capable of imaging nitrogen-vacancy centres with nanoscale resolution and Angstrom precision using focused light. The far-field optical control of the population of their excited state at the nanoscale expands the versatility of these centres and demonstrates the suitability of STED microscopy to image dense colour centres in crystals. Nitrogen-vacancy defects show great potential as tags for far-field optical nanoscopy(15) because they exhibit nearly ideal STED without bleaching. Measured point-spread functions of 5.8 nm in width demonstrate an all-physics-based far-field optical resolving power exceeding the wavelength of light by two orders of magnitude.
引用
收藏
页码:144 / 147
页数:4
相关论文
共 30 条
[1]  
[Anonymous], 1930, The Physical Principles of Quantum Theory
[2]   Nanoscale imaging magnetometry with diamond spins under ambient conditions [J].
Balasubramanian, Gopalakrishnan ;
Chan, I. Y. ;
Kolesov, Roman ;
Al-Hmoud, Mohannad ;
Tisler, Julia ;
Shin, Chang ;
Kim, Changdong ;
Wojcik, Aleksander ;
Hemmer, Philip R. ;
Krueger, Anke ;
Hanke, Tobias ;
Leitenstorfer, Alfred ;
Bratschitsch, Rudolf ;
Jelezko, Fedor ;
Wrachtrup, Joerg .
NATURE, 2008, 455 (7213) :648-U46
[3]   Imaging intracellular fluorescent proteins at nanometer resolution [J].
Betzig, Eric ;
Patterson, George H. ;
Sougrat, Rachid ;
Lindwasser, O. Wolf ;
Olenych, Scott ;
Bonifacino, Juan S. ;
Davidson, Michael W. ;
Lippincott-Schwartz, Jennifer ;
Hess, Harald F. .
SCIENCE, 2006, 313 (5793) :1642-1645
[4]   Single photon quantum cryptography [J].
Beveratos, A ;
Brouri, R ;
Gacoin, T ;
Villing, A ;
Poizat, JP ;
Grangier, P .
PHYSICAL REVIEW LETTERS, 2002, 89 (18) :1-187901
[5]   POSITION MEASUREMENT WITH A RESOLUTION AND NOISE-LIMITED INSTRUMENT [J].
BOBROFF, N .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1986, 57 (06) :1152-1157
[6]   Nanometer-sized diamond particle as a probe for biolabeling [J].
Chao, Jui-I. ;
Perevedentseva, Elena ;
Chung, Pei-Hua ;
Liu, Kuang-Kai ;
Cheng, Chih-Yuan ;
Chang, Chia-Ching ;
Cheng, Chia-Liang .
BIOPHYSICAL JOURNAL, 2007, 93 (06) :2199-2208
[7]   Macromolecular-scale resolution in biological fluorescence microscopy [J].
Donnert, Gerald ;
Keller, Jan ;
Medda, Rebecca ;
Andrei, M. Alexandra ;
Rizzoli, Silvio O. ;
Luehrmann, Reinhard ;
Jahn, Reinhard ;
Eggeling, Christian ;
Hell, Stefan W. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (31) :11440-11445
[8]   Focal spots of size λ/23 open up far-field florescence microscopy at 33 nm axial resolution -: art. no. 163901 [J].
Dyba, M ;
Hell, SW .
PHYSICAL REVIEW LETTERS, 2002, 88 (16) :4-163901
[9]   Fluorescence nanoscopy by ground-state depletion and single-molecule return [J].
Foelling, Jonas ;
Bossi, Mariano ;
Bock, Hannes ;
Medda, Rebecca ;
Wurm, Christian A. ;
Hein, Birka ;
Jakobs, Stefan ;
Eggeling, Christian ;
Hell, Stefan W. .
NATURE METHODS, 2008, 5 (11) :943-945
[10]   Characterization and application of single fluorescent nanodiamonds as cellular biomarkers [J].
Fu, Chi-Cheng ;
Lee, Hsu-Yang ;
Chen, Kowa ;
Lim, Tsong-Shin ;
Wu, Hsiao-Yun ;
Lin, Po-Keng ;
Wei, Pei-Kuen ;
Tsao, Pei-Hsi ;
Chang, Huan-Cheng ;
Fann, Wunshain .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (03) :727-732