Three-Dimensional Analysis of the Islet Vasculature

被引：24

作者：

El-Gohary, Y.

Sims-Lucas, S. ^{[2
]}

Lath, N. ^{[3
]}

Tulachan, S. ^{[4
]}

Guo, P.

Xiao, X.

Welsh, C.

Paredes, J.

Wiersch, J.

Prasadan, K.

Shiota, C.

Gittes, G. K. ^{[1
]}

机构：

[1] UPMC, Childrens Hosp Pittsburgh, Dept Pediat Surg, Div Pediat Gen & Thorac Surg, Pittsburgh, PA USA

[2] Univ Pittsburgh, Childrens Hosp Pittsburgh, Div Nephrol, Dept Pediat,Med Ctr, Pittsburgh, PA 15213 USA

[3] Morristown Mem Hosp, Dept Gen Surg, Morristown, NJ USA

[4] St Elizabeth Hlth Ctr, Dept Internal Med, Youngstown, OH USA

来源：

ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY | 2012年 / 295卷 / 09期

关键词：

islet; vasculature; pancreas; SCANNING-ELECTRON-MICROSCOPY; MURINE PANCREATIC-ISLETS; BETA-CELL MASS; ENDOTHELIAL-CELLS; IN-VIVO; EXOCRINE PANCREAS; BLOOD-VESSELS; MOUSE; VASCULARIZATION; ENDOCRINE;

D O I：

10.1002/ar.22530

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100123 [人体微生态学]; 100210 [外科学];

摘要：

The pancreatic islets of Langerhans are highly vascularized structures scattered throughout the pancreas that contain a capillary network 510 times denser than that of the exocrine pancreas. A simple method for three-dimensional (3D) analysis of this intricate intraislet vasculature has been difficult because of the intrinsic opacity of the pancreas. We developed a whole-mount imaging technique that allows relatively easy visualization of the islet vasculature. In combination with confocal microscopy and the use of 3D imaging software, we were able to readily reconstruct the 3D architecture of an islet, allowing delineation of the islet volume, length of the intraislet vessels, and the number of vessel branch-points. This technique allows for straightforward 3D image analysis that may help toward understanding islet function. Anat Rec, 2012. (C) 2012 Wiley Periodicals, Inc.

引用

页码：1473 / 1481

页数：9

共 44 条

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