Human herpesvirus 6 envelope cholesterol is required for virus entry

被引：52

作者：

Huang, HL

Li, YM

Sadaoka, T

Tang, HM

Yamamoto, T

Yamanishi, K

Mori, Y

机构：

[1] Osaka Univ, Grad Sch Med, Dept Microbiol, Suita, Osaka 5650871, Japan

[2] Natl Inst Biomed Innovat, Div Biomed Res, Lab Virol & Vaccinol, Ibaraki, Osaka 5670085, Japan

[3] Osaka Univ, Res Fdn Microbial Dis, Kagawa 7680061, Japan

来源：

JOURNAL OF GENERAL VIROLOGY | 2006年 / 87卷

关键词：

D O I：

10.1099/vir.0.81551-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

In this study, the role of cholesterol in the envelope of human herpesvirus 6 (HHV-6) was examined by using methyl-beta-cyclodextrin (M beta CD) depletion. When cholesterol was removed from HHV-6 virions with M beta CD, infectivity was abolished, but it could be rescued by the addition of exogenous cholesterol. HHV-6 binding was affected slightly by M beta CD treatment, In contrast, envelope cholesterol depletion markedly affected HHV-6 infectivity and HHV-6-induced cell fusion. These results suggest that the cholesterol present in the HHV-6 envelope plays a prominent role in the fusion process and is a key component in viral entry.

引用

收藏

页码：277 / 285

页数：9

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