The inhibition of Staphylococcus epidermidis biofilm formation by vancomycin-modified titanium alloy and implications for the treatment of periprosthetic infection

被引:193
作者
Antoci, Valentin, Jr. [1 ]
Adams, Christopher S. [1 ]
Parvizi, Javad [1 ,2 ]
Davidson, Helen M. [1 ]
Composto, Russell J. [3 ,4 ]
Freeman, Theresa A. [1 ]
Wickstrom, Eric [5 ]
Ducheyne, Paul [3 ,6 ]
Jungkind, Donald [7 ]
Shapiro, Irving M. [1 ,3 ,5 ]
Hickok, Noreen J. [1 ,5 ]
机构
[1] Thomas Jefferson Univ, Dept Orthopaed Surg, Philadelphia, PA 19107 USA
[2] Rothman Inst, Philadelphia, PA USA
[3] Univ Penn, Sch Engn & Appl Sci, Ctr Bioact Mat & Tissue Engn, Philadelphia, PA 19104 USA
[4] Univ Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USA
[5] Thomas Jefferson Univ, Dept Biochem & Mol Biol, Philadelphia, PA 19107 USA
[6] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA
[7] Thomas Jefferson Univ, Dept Pathol Anat & Cell Biol, Philadelphia, PA 19107 USA
关键词
Antibacterial; Bacterial adhesion; Biofilm; Metal surface treatment; Bacteria; Surface modification; Titanium alloy;
D O I
10.1016/j.biomaterials.2008.08.016
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Peri-prosthetic infections are notoriously difficult to treat as the biomaterial implant is ideal for bacterial adhesion and biofilm formation, resulting in decreased antibiotic sensitivity. Previously, we reported that vancomycin covalently attached to a Ti alloy surface (Vanc-Ti) could prevent bacterial colonization. Herein we examine the effect of this Vanc-Ti surface on Staphylococci epidermidis, a Gram-positive organism prevalent in orthopaedic infections. By direct colony counting and fluorescent visualization of live bacteria, S. epidermidis colonization was significantly inhibited on Vanc-Ti implants. In contrast, the gram-negative organism Escherichia coli readily colonized the Vanc-Ti rod, suggesting retention of antibiotic specificity. By histochemical and SEM analysis, Vanc-Ti prevented S. epidermidis biofilm formation, even in the presence of serum. Furthermore, when challenged multiple times with S. epidermidis, Vanc-Ti rods resisted bacterial colonization. Finally, when S. epidermidis was continuously cultured in the presence of Vanc-Ti, the bacteria maintained a Vanc sensitivity equivalent to the parent strain. These findings indicate that antibiotic derivatization of implants can result in a surface that can resist bacterial colonization. This technology holds great promise for the prevention and treatment of periprosthetic infections. (c) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4684 / 4690
页数:7
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