Antibody-labeled fluorescence imaging of dendritic cell populations in vivo

被引：18

作者：

Cummings, Ryan J. ^{[2
]}

Mitra, Soumya ^{[1
]}

Lord, Edith M. ^{[2
]}

Foster, Thomas H. ^{[1
]}

机构：

[1] Univ Rochester, Med Ctr, Dept Imaging Sci, Rochester, NY 14642 USA

[2] Univ Rochester, Med Ctr, Dept Microbiol & Immunol, Rochester, NY 14642 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2008年 / 13卷 / 04期

关键词：

in vivo imaging; immune cell imaging; confocal microscopy;

D O I：

10.1117/1.2966122

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We report an optical molecular imaging technique that exploits local administration of fluorophore-conjugated antibodies and confocal fluorescence microscopy to achieve high-contrast imaging of host cell populations in normal and tumor tissue in living mice. The method achieves micron-scale spatial resolution to depths greater than 100 mu m. We illustrate the capabilities of this approach by imaging two dendritic cell populations in the skin and normal and tumor vasculature in vivo. (C) 2008 Society of Photo-Optical Instrumentation Engineers.

引用

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