Experimental aspects of biological X-ray absorption spectroscopy

被引：52

作者：

Ascone, I

Meyer-Klaucke, W

Murphy, L

机构：

[1] Univ Paris 11, LURE, F-91898 Orsay, France

[2] DESY, EMBL, D-22607 Hamburg, Germany

[3] Daresbury Lab, Warrington WA4 4AD, Cheshire, England

来源：

JOURNAL OF SYNCHROTRON RADIATION | 2003年 / 10卷

关键词：

BioXAS; metalloproteins; experimental set-up; macromolecules;

D O I：

10.1107/S0909049502022598

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Spectroscopic techniques, like X-ray absorption spectroscopy, will provide important input for integrated biological projects in genomics and proteomics. This contribution summarizes technical requirements and typical set-ups for both simple and complex biological XAS experiments. An overview on different strategies for sample preparation is discussed in detail. Present and future BioXAS spectrometers are presented to help potential users in locating the spectrometer required for their biological application.

引用

页码：16 / 22

页数：7

共 52 条

[1] Saccharose solid matrix embedded proteins: a new method for sample preparation for X-ray absorption spectroscopy
Ascone, I
Sabatucci, A
Bubacco, L
Di Muro, P
Salvato, B
[J]. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2000, 29 (06): : 391 - 397
[2] X-ray absorption spectroscopy and electrochemistry on biological samples
Ascone, I
Cognigni, A
Giorgetti, M
Berrettoni, M
Zamponi, S
Marassi, R
[J]. JOURNAL OF SYNCHROTRON RADIATION, 1999, 6 : 384 - 386
[3] Evidence of His61 imidazolate bridge rupture in reduced crystalline Cu,Zn superoxide dismutase
Ascone, I
Castañer, R
Tarricone, C
Bolognesi, M
Stroppolo, ME
Desideri, A
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 241 (01) : 119 - 121
[4] Ascone I, 2000, HIGH PRESSURE RES, V19, P277, DOI 10.1080/08957950008202565
[5] BANLY C, 2002, BIOCHIM BIOPHYS ACTA, V1595, P3
[6] Pressure effects on in vivo microbial processes
Bartlett, DH
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 2002, 1595 (1-2): : 367 - 381
[7] THE CO BOND ANGLE OF CARBOXYMYOGLOBIN DETERMINED BY ANGULAR-RESOLVED XANES SPECTROSCOPY
BIANCONI, A
CONGIUCASTELLANO, A
DURHAM, PJ
HASNAIN, SS
PHILLIPS, S
[J]. NATURE, 1985, 318 (6047) : 685 - 687
[8] CHEN J, 1993, ANGEW CHEM INT EDIT, V32, P1592
[9] Determination of iron-ligand bond lengths in ferric and ferrous horse heart cytochrome c using multiple scattering analyses of XAFS data
Cheng, MC
Rich, AM
Armstrong, RS
Ellis, PJ
Lay, PA
[J]. INORGANIC CHEMISTRY, 1999, 38 (25) : 5703 - 5708
[10] A quasi-solid state electrochemical cell for in situ EXAFS measurements on biological samples
Cognigni, A
Ascone, I
Zamponi, S
Marassi, R
[J]. JOURNAL OF SYNCHROTRON RADIATION, 2001, 8 (02) : 987 - 989

← 1 2 3 4 5 6 →