Evidence of His61 imidazolate bridge rupture in reduced crystalline Cu,Zn superoxide dismutase

被引：24

作者：

Ascone, I

Castañer, R

Tarricone, C

Bolognesi, M

Stroppolo, ME

Desideri, A

机构：

[1] Univ Paris 11, Utilisat Rayonnement Electromagnet Lab, Fac Orsay, F-91405 Orsay, France

[2] Univ Pavia, Dip Genet & Microbiol, I-27100 Pavia, Italy

[3] Univ Genoa, INFM, I-16146 Genoa, Italy

[4] Univ Genoa, Ctr Biotecnol Avanzate, I-16146 Genoa, Italy

[5] Univ Rome Tor Vergata, Dept Biol, I-00173 Rome, Italy

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 241卷 / 01期

关键词：

bovine Cu; Zn superoxide dismutase; XAS; copper co-ordination; redox states; bridging ligands;

D O I：

10.1006/bbrc.1997.7777

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

X-ray absorption spectroscopy has been carried out on the copper K edge in oxidized and reduced bovine Cu,Zn SOD in solution and in crystalline state. The results indicate that the copper coordination geometry is unaffected by the solution or by the crystalline state of the protein, in both oxidation states. Moreover the two oxidation states of the active copper ion are reflected under, all the experimental conditions, by distinct coordination spheres around the catalytic metal, which is four-coordinated and three-coordinated in the Cu(II) and in the Cu(I) enzyme, respectively. (C) 1997 Academic Press.

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收藏

页码：119 / 121

页数：3

相关论文

共 18 条

[1] X-RAY, NMR AND MOLECULAR-DYNAMICS STUDIES ON REDUCED BOVINE SUPEROXIDE-DISMUTASE - IMPLICATIONS FOR THE MECHANISM [J].

BANCI, L ;

BERTINI, I ;

BRUNI, B ;

CARLONI, P ;

LUCHINAT, C ;

MANGANI, S ;

ORIOLI, PL ;

PICCIOLI, M ;

RYPNIEWSKI, W ;

WILSON, KS .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 202 (02) :1088-1095

[2] ASPECTS OF THE STRUCTURE, FUNCTION, AND APPLICATIONS OF SUPEROXIDE-DISMUTASE [J].

BANNISTER, JV ;

BANNISTER, WH ;

ROTILIO, G .

CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1987, 22 (02) :111-180

[3] EVIDENCE OF THE BREAKING OF THE COPPER IMIDAZOLATE BRIDGE IN COPPER COBALT-SUBSTITUTED SUPEROXIDE-DISMUTASE UPON REDUCTION OF THE COPPER(II) CENTERS [J].

BERTINI, I ;

LUCHINAT, C ;

MONNANNI, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (07) :2178-2179

[4] AN EXTENDED-X-RAY-ABSORPTION-FINE-STRUCTURE STUDY OF BOVINE ERYTHROCYTE SUPEROXIDE-DISMUTASE IN AQUEOUS-SOLUTION - DIRECT EVIDENCE FOR 3-CO-ORDINATE CU(I) IN REDUCED ENZYME [J].

BLACKBURN, NJ ;

HASNAIN, SS ;

BINSTED, N ;

DIAKUN, GP ;

GARNER, CD ;

KNOWLES, PF .

BIOCHEMICAL JOURNAL, 1984, 219 (03) :985-990

[5] ANION BINDING TO BOVINE ERYTHROCYTE SUPEROXIDE-DISMUTASE STUDIED BY X-RAY ABSORPTION-SPECTROSCOPY - A DETAILED STRUCTURAL-ANALYSIS OF THE NATIVE ENZYME AND THE AZIDO AND CYANO DERIVATIVES USING A MULTIPLE-SCATTERING APPROACH [J].

BLACKBURN, NJ ;

STRANGE, RW ;

MCFADDEN, LM ;

HASNAIN, SS .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1987, 109 (23) :7162-7170

[6] CONSERVED PATTERNS IN THE CU,ZN SUPEROXIDE-DISMUTASE FAMILY [J].

BORDO, D ;

DJINOVIC, K ;

BOLOGNESI, M .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 238 (03) :366-386

[7] SOFT-X-RAY SPECTROSCOPY OF METALLOPROTEINS USING FLUORESCENCE DETECTION [J].

CRAMER, SP ;

CHEN, J ;

GEORGE, SJ ;

VANELP, J ;

MOORE, J ;

TENSCH, O ;

COLARESI, J ;

YOCUM, M ;

MULLINS, OC ;

CHEN, CT .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 1992, 319 (1-3) :285-289

[8] LOW-TEMPERATURE OPTICAL SPECTROSCOPY OF NATIVE AND AZIDE-REACTED BOVINE CU,ZN SUPEROXIDE-DISMUTASE - A STRUCTURAL DYNAMICS STUDY [J].

CUPANE, A ;

LEONE, M ;

MILITELLO, V ;

STROPPOLO, ME ;

POLTICELLI, F ;

DESIDERI, A .

BIOCHEMISTRY, 1994, 33 (50) :15103-15109

[9] CRYSTAL-STRUCTURE SOLUTION AND REFINEMENT OF THE SEMISYNTHETIC COBALT-SUBSTITUTED BOVINE ERYTHROCYTE SUPEROXIDE-DISMUTASE AT 2.0 ANGSTROM RESOLUTION [J].

DJINOVIC, K ;

CODA, A ;

ANTOLINI, L ;

PELOSI, G ;

DESIDERI, A ;

FALCONI, M ;

ROTILIO, G ;

BOLOGNESI, M .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 226 (01) :227-238

[10] DEVELOPMENT OF A NEW ENERGY SCANNED (4-20 KEV) EXAFS-II SPECTROMETER AT LURE [J].

GOULON, J ;

LEMONNIER, M ;

CORTES, R ;

RETOURNARD, A ;

RAOUX, D .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH, 1983, 208 (1-3) :625-630