Oligosaccharlde (OS) production, cell concentration (2x10(9) colony-forming unit/ml), lactose concentration (25% wt/vol), reaction time (6 h), and temperature (50degreesC) were chosen as the central condition of the central composite design (CCD) for optimizing the production process using Bifidobacterium intantis RW-8120 in skim milk. Statistical analysis (P < 0.01) revealed that the most relevant variable concerning OS production and yield was the lactose concentration. The coefficient of determination (R-2) is good for the second-order OS production model (0.92) and fairly good for the second-order nonlinear OS yield model (0.816). An increase of lactose concentration and temperature resulted in a higher OS production. The optimal values for OS production appear to be near the area associated with the central points of the modeling design except for the lactose concentration, which was 40% (wt/vol) of the final volume.
机构:
Univ Catolica Portuguesa Rua, Escola Super Biotecnol, P-4200 Porto, PortugalUniv Catolica Portuguesa Rua, Escola Super Biotecnol, P-4200 Porto, Portugal
Gomes, AMP
;
Malcata, FX
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机构:
Univ Catolica Portuguesa Rua, Escola Super Biotecnol, P-4200 Porto, PortugalUniv Catolica Portuguesa Rua, Escola Super Biotecnol, P-4200 Porto, Portugal
机构:
Univ Catolica Portuguesa Rua, Escola Super Biotecnol, P-4200 Porto, PortugalUniv Catolica Portuguesa Rua, Escola Super Biotecnol, P-4200 Porto, Portugal
Gomes, AMP
;
Malcata, FX
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h-index: 0
机构:
Univ Catolica Portuguesa Rua, Escola Super Biotecnol, P-4200 Porto, PortugalUniv Catolica Portuguesa Rua, Escola Super Biotecnol, P-4200 Porto, Portugal