Optimization of galacto-oligosaccharide production by Bifidobacterium infantis RW-8120 using response surface methodology

被引:33
作者
Roy, D
Daoudi, L
Azaola, A
机构
[1] Agr & Agri Food Canada, Ctr Food Res & Dev, St Hyacinthe, PQ J2S 8E3, Canada
[2] Univ Autonoma Metropolitana, Dept Sistemas Biol, Mexico City 16000, DF, Mexico
关键词
prebiotic; probiotic strains; bifidobacteria; central composite design; galacto-oligosaccharides; lactose concentration; optimization;
D O I
10.1038/sj.jim.7000319
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Oligosaccharlde (OS) production, cell concentration (2x10(9) colony-forming unit/ml), lactose concentration (25% wt/vol), reaction time (6 h), and temperature (50degreesC) were chosen as the central condition of the central composite design (CCD) for optimizing the production process using Bifidobacterium intantis RW-8120 in skim milk. Statistical analysis (P < 0.01) revealed that the most relevant variable concerning OS production and yield was the lactose concentration. The coefficient of determination (R-2) is good for the second-order OS production model (0.92) and fairly good for the second-order nonlinear OS yield model (0.816). An increase of lactose concentration and temperature resulted in a higher OS production. The optimal values for OS production appear to be near the area associated with the central points of the modeling design except for the lactose concentration, which was 40% (wt/vol) of the final volume.
引用
收藏
页码:281 / 285
页数:5
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