Detection of individual p53-autoantibodies by using quenched peptide-based molecular probes

被引：53

作者：

Neuweiler, H

Schulz, A

Vaiana, AC

Smith, JC

Kaul, S

Wolfrum, J

Sauer, M

机构：

[1] Univ Heidelberg, Inst Chem Phys, D-69120 Heidelberg, Germany

[2] Univ Heidelberg, IWR, D-69120 Heidelberg, Germany

[3] Univ Frauenklin Heidelberg, D-69115 Heidelberg, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2002年 / 41卷 / 24期

关键词：

D O I：

10.1002/anie.200290044

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Single-molecule spectroscopy for cancer diagnostics: Short dye-labeled peptide epitopes derived from human p53 show strong fluorescence quenching owing to efficient electron transfer from a tryptophan residue to the fluorophore (1, see picture). The specific recognition of the peptide epitope by the antibody induces a change in the conformation which is accompanied by a strong increase in fluorescence intensity. The fluorescing antibody - peptide complex can be detected at the single-molecule level even in slightly diluted serum samples by using confocal fluorescence microscopy.

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页码：4769 / 4773

页数：5

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