PROBING INDIVIDUAL MOLECULES WITH CONFOCAL FLUORESCENCE MICROSCOPY

被引：517

作者：

NIE, SM ^{[1
]}

CHIU, DT ^{[1
]}

ZARE, RN ^{[1
]}

机构：

[1] STANFORD UNIV,DEPT CHEM,STANFORD,CA 94305

来源：

SCIENCE | 1994年 / 266卷 / 5187期

关键词：

D O I：

10.1126/science.7973650

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Confocal fluorescence microscopy coupled with a diffraction-limited laser beam and a high-efficiency detection system has been used to study the diffusive movement and emission process of individual fluorescent molecules in the liquid phase at room temperature. The high detection sensitivity achieved at fast data acquisition speeds (greater than 1 kilohertz) allows real-time observation of single-molecule fluorescence without statistical analysis. The results show fluorescence-cycle saturation at the single-molecule level and multiple recrossings of a single molecule into and out of the probe volume as well as the triplet state.

引用

页码：1018 / 1021

页数：4

共 36 条

[1] ALTERATIONS OF SINGLE-MOLECULE FLUORESCENCE LIFETIMES IN NEAR-FIELD OPTICAL MICROSCOPY [J].