Structural and functional analysis of hypothetical proteins in mouse hippocampus from two-dimensional gel electrophoresis

被引：9

作者：

Afjehi-Sadat, Leila

Yang, Jae-Won

Pollak, Arnold

Kim, Dae-Won

Choi, Soo-Young

Lubec, Gert

机构：

[1] Med Univ Vienna, Dept Pediat, A-1090 Vienna, Austria

[2] Hallym Univ, Dept Biomed Sci, Chunchon, South Korea

来源：

JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 02期

关键词：

hypothetical proteins; enzyme activity; 2D gel electrophoresis; recovery of enzyme activity; mass spectrometry;

D O I：

10.1021/pr060453o

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein profiling in five individual mouse strains showed strain-specific expression of three hypothetical proteins (HPs). As functional and structural assignment of HPs were based on predictions and low identity to known structures, HPs were identified by MALDI-TOF/TOF, and their proposed tentative function was determined by enzyme assays. Three identified HPs were extracted from gels and renatured, and pyridoxal phosphate phosphatase, inorganic pyrophosphate phosphatase, and antioxidant activities were revealed, findings in agreement with functional predictions.

引用

收藏

页码：711 / 723

页数：13

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