Expression of interleukin-6 and its effect on the cell growth of gastric carcinoma cell lines

被引：36

作者：

Ito, R

Yasui, W

Kuniyasu, H

Yokozaki, H

Tahara, E

机构：

[1] HIROSHIMA UNIV,SCH MED,DEPT PATHOL 1,MINAMI KU,HIROSHIMA 734,JAPAN

[2] OSAKA UNIV,FAC DENT,DEPT ORAL PATHOL,SUITA,OSAKA 565,JAPAN

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1997年 / 88卷 / 10期

关键词：

IL-6; gastric carcinoma cell line; autocrine; cytokine;

D O I：

10.1111/j.1349-7006.1997.tb00314.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The expression and the effect of IL-6 were examined in human gastric carcinoma cell lines to determine whether IL-6 serves as a growth stimulator. The expression of IL-6 mRNA was detected in three (TMK-1, MKN-1, MKN-7) of 8 gastric carcinoma cell lines. All three cell lines secreted IL-6 into the culture fluid, in large amounts in the cases of MKN-1 and MKN-7 cells. Scatchard plot analysis of IL-6 binding revealed that MKN-1 and MKN-7 cells had both high-and low-affinity receptors. Cell growth of MKN-1 and MKN-7 cells was stimulated by IL-6, while anti-IL-6 antibody inhibited growth. The expression of IL-la mRNA by these three cell lines was induced by IL-6. IL-1 alpha increased the expression of mRNA for IL-6 by TMK-1 cells. These findings indicate that IL-6 induced by IL-1 alpha is an autocrine growth factor for some gastric carcinomas.

引用

页码：953 / 958

页数：6

共 30 条

[1] BIOLOGIC EFFECTS OF ANTI-INTERLEUKIN-6 MURINE MONOCLONAL-ANTIBODY IN ADVANCED MULTIPLE-MYELOMA [J].