Heterotypic Humoral and Cellular Immune Responses following Norwalk Virus Infection
被引:103
作者:
Lindesmith, Lisa C.
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机构:Univ N Carolina, Sch Publ Hlth, Dept Epidemiol, Chapel Hill, NC 27599 USA
Lindesmith, Lisa C.
Donaldson, Eric
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h-index: 0
机构:
Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USAUniv N Carolina, Sch Publ Hlth, Dept Epidemiol, Chapel Hill, NC 27599 USA
Donaldson, Eric
[2
]
Leon, Juan
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机构:
Emory Univ, Hubert Dept Global Hlth, Atlanta, GA 30322 USAUniv N Carolina, Sch Publ Hlth, Dept Epidemiol, Chapel Hill, NC 27599 USA
Leon, Juan
[3
]
Moe, Christine L.
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机构:
Emory Univ, Hubert Dept Global Hlth, Atlanta, GA 30322 USAUniv N Carolina, Sch Publ Hlth, Dept Epidemiol, Chapel Hill, NC 27599 USA
Moe, Christine L.
[3
]
Frelinger, Jeffrey A.
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机构:
Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USAUniv N Carolina, Sch Publ Hlth, Dept Epidemiol, Chapel Hill, NC 27599 USA
Frelinger, Jeffrey A.
[2
]
Johnston, Robert E.
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机构:
Carolina Vaccine Inst, Chapel Hill, NC USAUniv N Carolina, Sch Publ Hlth, Dept Epidemiol, Chapel Hill, NC 27599 USA
Johnston, Robert E.
[4
]
Weber, David J.
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机构:
Univ N Carolina, Dept Med, Chapel Hill, NC 27599 USAUniv N Carolina, Sch Publ Hlth, Dept Epidemiol, Chapel Hill, NC 27599 USA
Weber, David J.
[5
]
Baric, Ralph S.
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机构:
Univ N Carolina, Sch Publ Hlth, Dept Epidemiol, Chapel Hill, NC 27599 USA
Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA
Carolina Vaccine Inst, Chapel Hill, NC USAUniv N Carolina, Sch Publ Hlth, Dept Epidemiol, Chapel Hill, NC 27599 USA
Baric, Ralph S.
[1
,2
,4
]
机构:
[1] Univ N Carolina, Sch Publ Hlth, Dept Epidemiol, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA
[3] Emory Univ, Hubert Dept Global Hlth, Atlanta, GA 30322 USA
[4] Carolina Vaccine Inst, Chapel Hill, NC USA
[5] Univ N Carolina, Dept Med, Chapel Hill, NC 27599 USA
BLOOD GROUP ANTIGENS;
REVERSE-TRANSCRIPTION-PCR;
CAPSID PROTEIN FORMS;
ACUTE GASTROENTERITIS;
MONOCLONAL-ANTIBODIES;
GENOGROUP-I;
MOLECULAR CHARACTERIZATION;
GENETIC DIVERSITY;
HUMAN NOROVIRUS;
SECRETOR FUT2;
D O I:
10.1128/JVI.02179-09
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Norovirus immunity is poorly understood as the limited data available on protection after infection are often contradictory. In contrast to the more prominent GII noroviruses, GI norovirus infections are less frequent in outbreaks. The GI noroviruses display very complex patterns of heterotypic immune responses following infection, and many individuals are highly susceptible to reinfection. To study the immune responses and mechanisms of GI. 1 persistence, we built structural models and recombinant virus-like particles (VLPs) of five GI strains: GI. 1-1968, GI. 1-2001, GI. 2-1999, GI. 3-1999, and GI. 4-2000. Structural models of four GI genotype capsid P domain dimers suggested that intragenotype structural variation is limited, that the GI binding pocket is mostly preserved between genotypes, and that a conserved, surface-exposed epitope may allow for highly cross-reactive immune responses. GI VLPs bound to histo-blood group antigens (HBGAs) including fucose, Lewis, and A antigens. Volunteers infected with GI. 1-1968 (n = 10) had significant increases between prechallenge and convalescent reactive IgG for all five GI VLPs measured by enzyme immunoassay. Potential cross-neutralization of GI VLPs was demonstrated by convalescent-phase serum cross-blockade of GI VLP-HBGA interaction. Although group responses were significant for all GI VLPs, each individual volunteer demonstrated a unique VLP blockade pattern. Further, peripheral blood mononuclear cells (PBMCs) were stimulated with each of the VLPs, and secretion of gamma interferon (IFN-gamma) was measured. As seen with blockade responses, IFN-gamma secretion responses differed by individual. Sixty percent responded to at least one GI VLP, with only two volunteers responding to GI. 1 VLP. Importantly, four of five individuals with sufficient PBMCs for cross-reactivity studies responded more robustly to other GI VLPs. These data suggest that preexposure history and deceptive imprinting may complicate PBMC and B-cell immune responses in some GI. 1-1968-challenged individuals and highlight a potential complication in the design of efficacious norovirus vaccines.
机构:
Gartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, ScotlandGartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, Scotland
Adamson, W. E.
Gunson, R. N.
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机构:
Gartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, ScotlandGartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, Scotland
Gunson, R. N.
Maclean, A.
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h-index: 0
机构:
Gartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, ScotlandGartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, Scotland
Maclean, A.
Carman, W. F.
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h-index: 0
机构:
Gartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, ScotlandGartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, Scotland
机构:
Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USAUniv N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Baric, RS
Yount, B
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机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Yount, B
Lindesmith, L
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机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Lindesmith, L
Harrington, PR
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机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Harrington, PR
Greene, SR
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机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Greene, SR
Tseng, FC
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机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Tseng, FC
Davis, N
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h-index: 0
机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Davis, N
Johnston, RE
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机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Johnston, RE
Klapper, DG
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机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Klapper, DG
Moe, CL
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机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
机构:
Gartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, ScotlandGartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, Scotland
Adamson, W. E.
Gunson, R. N.
论文数: 0引用数: 0
h-index: 0
机构:
Gartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, ScotlandGartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, Scotland
Gunson, R. N.
Maclean, A.
论文数: 0引用数: 0
h-index: 0
机构:
Gartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, ScotlandGartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, Scotland
Maclean, A.
Carman, W. F.
论文数: 0引用数: 0
h-index: 0
机构:
Gartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, ScotlandGartnavel Royal Hosp, W Scotland Special Virol Ctr, Glasgow G12 OYN, Lanark, Scotland
机构:
Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USAUniv N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Baric, RS
Yount, B
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h-index: 0
机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Yount, B
Lindesmith, L
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机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Lindesmith, L
Harrington, PR
论文数: 0引用数: 0
h-index: 0
机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Harrington, PR
Greene, SR
论文数: 0引用数: 0
h-index: 0
机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Greene, SR
Tseng, FC
论文数: 0引用数: 0
h-index: 0
机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Tseng, FC
Davis, N
论文数: 0引用数: 0
h-index: 0
机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Davis, N
Johnston, RE
论文数: 0引用数: 0
h-index: 0
机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Johnston, RE
Klapper, DG
论文数: 0引用数: 0
h-index: 0
机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA
Klapper, DG
Moe, CL
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h-index: 0
机构:Univ N Carolina, Program Infect Dis, Dept Epidemiol, Sch Publ Hlth, Chapel Hill, NC 27599 USA