Expression of the Vanin Gene Family in Normal and Inflamed Human Skin: Induction by Proinflammatory Cytokines

被引：69

作者：

Jansen, Patrick A. M. ^{[1
,2
]}

Kamsteeg, Marijke ^{[1
,2
]}

Rodijk-Olthuis, Diana ^{[1
,2
]}

van Vlijmen-Willems, Ivonne M. J. J. ^{[1
,2
]}

de Jongh, Gys J. ^{[1
,2
]}

Bergers, Mieke ^{[1
,2
]}

Tjabringa, Geuranne S. ^{[1
,2
]}

Zeeuwen, Patrick L. J. M. ^{[1
,2
]}

Schalkwijk, Joost ^{[1
,2
]}

机构：

[1] Radboud Univ Nijmegen, Med Ctr, Dept Dermatol, NL-6525 ED Nijmegen, Netherlands

[2] Radboud Univ Nijmegen, Med Ctr, Nijmegen Ctr Mol Life Sci, NL-6525 ED Nijmegen, Netherlands

来源：

JOURNAL OF INVESTIGATIVE DERMATOLOGY | 2009年 / 129卷 / 09期

关键词：

ACTIVATED-RECEPTOR-GAMMA; HUMAN EPIDERMAL-KERATINOCYTES; ATOPIC-DERMATITIS; TRANSGLUTAMINASE ACTIVITY; PSORIATIC SKIN; DIFFERENTIATION; PANTETHEINASE; DISEASE; MOUSE; LOCALIZATION;

D O I：

10.1038/jid.2009.67

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

The vanin gene family encodes secreted and membrane-bound ectoenzymes that convert pantetheine into pantothenic acid and cysteamine. Recent studies in a mouse colitis model indicated that vanin-1 has proinflammatory activity and suggest that pantetheinases are potential therapeutic targets in inflammatory diseases. In a microarray analysis of epidermal gene expression of psoriasis and atopic dermatitis lesions, we identified vanin-3 as the gene showing the highest differential expression of all annotated genes that we studied (19-fold upregulation in psoriasis). Quantitative real-time PCR analysis confirmed the microarray data on vanin-3 and showed similar induction of vanin-1, but not of vanin-2, in psoriatic epidermis. Immunohistochemistry showed that vanin-3 is expressed in the differentiated epidermal layers. Using submerged and organotypic keratinocyte cultures, we found that vanin-1 and vanin-3 are induced at the mRNA and protein level by psoriasis-associated proinflammatory cytokines (Th17/Th1) but not by Th2 cytokines. We hypothesize that increased levels of pantetheinase activity are part of the inflammatory-regenerative epidermal differentiation program, and may contribute to the phenotype observed in psoriasis.

引用

页码：2167 / 2174

页数：8

共 51 条

[11] INCREASED MEMBRANE-ASSOCIATED TRANSGLUTAMINASE ACTIVITY IN PSORIASIS [J].

ESMANN, J ;

VOORHEES, JJ ;

FISHER, GJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 164 (01) :219-224

[12] Two human genes related to murine Vanin-1 are located on the long arm of human chromosome 6 [J].

Galland, F ;

Malergue, F ;

Bazin, H ;

Mattei, MG ;

Aurrand-Lions, M ;

Theillet, C ;

Naquet, P .

GENOMICS, 1998, 53 (02) :203-213

[13] An ESTs description of the new Vanin gene family conserved from fly to human [J].

Granjeaud, S ;

Naquet, P ;

Galland, F .

IMMUNOGENETICS, 1999, 49 (11-12) :964-972

[14] SKIN WATER LOSS AND ACCIDENTAL HYPOTHERMIA IN PSORIASIS ICHTHYOSIS AND ERYTHRODERMA [J].

GRICE, KA ;

BETTLEY, FR .

BRITISH MEDICAL JOURNAL, 1967, 4 (5573) :195-&

[15] Transglutaminase inhibitors induce hyperproliferation and parakeratosis in tissue-engineered skin [J].

Harrison, C. A. ;

Layton, C. M. ;

Hau, Z. ;

Bullock, A. J. ;

Johnson, T. S. ;

MacNeil, S. .

BRITISH JOURNAL OF DERMATOLOGY, 2007, 156 (02) :247-257

[16] Psoriasis is associated with increased β-defensin genomic copy number [J].

Hollox, Edward J. ;

Huffmeier, Ulrike ;

Zeeuwen, Patrick L. J. M. ;

Palla, Raquel ;

Lascorz, Jesús ;

Rodijk-Olthuis, Diana ;

van de Kerkhof, Peter C. M. ;

Traupe, Heiko ;

de Jongh, Gys ;

den Heijer, Martin ;

Reis, Andre ;

Armour, John A. L. ;

Schalkwijk, Joost .

NATURE GENETICS, 2008, 40 (01) :23-25

[17] Different inhibition characteristics of intracellular transglutaminase activity by cystamine and cysteamine [J].

Jeon, JH ;

Lee, HJ ;

Jang, GY ;

Kim, CW ;

Shin, DM ;

Cho, SY ;

Yeo, EJ ;

Park, SC ;

Kim, IG .

EXPERIMENTAL AND MOLECULAR MEDICINE, 2004, 36 (06) :576-581

[18] Increased expression of carbonic anhydrase II (CA II) in lesional skin of atopic dermatitis: Regulation by Th2 cytokines [J].

Kamsteeg, Marijke ;

Zeeuwen, Patrick L. J. M. ;

de Jongh, Gys J. ;

Rodijk-Olthuis, Diana ;

Zeeuwen-Franssen, Manon E. J. ;

van Erp, Piet E. J. ;

Schalkwijk, Joost .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2007, 127 (07) :1786-1789

[19]

KIM IG, 1993, J BIOL CHEM, V268, P12682

[20]

Le M, 1996, ARCH DERMATOL RES, V288, P684, DOI 10.1007/BF02505278

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