Triacylglycerol mixture for testing capillary columns for high-temperature gas chromatography

The quality of fused-silica capillary columns was investigated with a mixture of triacylglycerols at high temperatures. The molecular mass of the triacylglycerols ranges between 800 and 1140 and the elution temperatures are between 300 and 420 degrees C. Essential properties of the capillary columns, such as separation power, selectivity, catalytic degradation, adsorption and bleeding were determined at high temperatures. Capillary columns coated with the five widely-used stationary phases OV-1, SE-52, OV-1701-OH, SOP-50 and OV-25 were checked. Elution patterns and information about separation power and degradation of the triacylglycerols are given. A simple method was elaborated to determine the column bleeding.