Biosensor for phenol based on the direct electron transfer blocking of peroxidase immobilising on silica-titanium

被引：114

作者：

Rosatto, SS ^{[1
]}

Kubota, LT ^{[1
]}

Neto, GD ^{[1
]}

机构：

[1] UNICAMP, Inst Quim, BR-13083970 Campinas, SP, Brazil

来源：

ANALYTICA CHIMICA ACTA | 1999年 / 390卷 / 1-3期

基金：

巴西圣保罗研究基金会;

关键词：

modified silica; phenol biosensor; peroxidase;

D O I：

10.1016/S0003-2670(99)00168-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Horseradish peroxidase (HRP) was immobilised on silica gel modified with titanium oxide. This material was employed to prepare modified carbon paste electrode. The direct electron transfer of the hydrogen peroxide reduction by HRP was blocked when immobilised on silica-titanium, This biosensor presented a very sensitive response for phenol (1 mu mol l(-1)) at an applied potential of 0 mV vs SCE. The best condition was achieved in phosphate buffer pH 6.8, ratio of hydrogen peroxide/phenol higher than 0.35. The biosensor showed a linear response range between 10 and 50 mu mol l(-1) of phenol, adjusted by the equation j=-32.8+16.3 [phenol], for n=5 with a correlation coefficient of 0.9995. The response time of the biosensor was about 3 s. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：65 / 72

页数：8

共 31 条

[11] CYCLIC VOLTAMMETRIC STUDY OF [FE(CN)6]3-/4- IMMOBILIZED ON SILICA-GEL SURFACE COATED WITH TITANIUM(IV) OXIDE [J].