Auxin transport through non-hair cells sustains root-hair development

被引:180
作者
Jones, Angharad R. [1 ]
Kramer, Eric M. [2 ,3 ]
Knox, Kirsten [4 ,5 ]
Swarup, Ranjan [6 ]
Bennett, Malcolm J. [6 ]
Lazarus, Colin M. [1 ]
Leyser, H. M. Ottoline [4 ]
Grierson, Claire S. [1 ]
机构
[1] Univ Bristol, Sch Biol Sci, Bristol BS8 1UG, Avon, England
[2] Bard Coll Simons Rock, Dept Phys, Massachusetts, MA 01230 USA
[3] Univ Nottingham, Ctr Plant Integrat Biol, Loughborough LE12 5RD, Leics, England
[4] Univ York, Dept Biol, York YO10 5YW, N Yorkshire, England
[5] Univ Edinburgh, Inst Mol Plant Sci, Edinburgh EH9 3JR, Midlothian, Scotland
[6] Univ Nottingham, Sch Biosci, Plant Sci Div, Loughborough LE12 5RD, Leics, England
基金
英国生物技术与生命科学研究理事会;
关键词
LATERAL ROOT; ARABIDOPSIS; EFFLUX; PIN; TRAFFICKING; POLARITY; LOCALIZATION; INFORMATION; ELONGATION; EPIDERMIS;
D O I
10.1038/ncb1815
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The plant hormone auxin controls root epidermal cell development in a concentration-dependent manner(1-3). Root hairs are produced on a subset of epidermal cells as they increase in distance from the root tip. Auxin is required for their initiation(4-7) and continued growth(8-11), but little is known about its distribution in this region of the root. Contrary to the expectation that hair cells might require active auxin influx to ensure auxin supply, we did not detect the auxin-influx transporter AUX1 in root-hair cells. A high level of AUX1 expression was detected in adjacent non-hair cell files. Non-hair cells were necessary to achieve wild-type root-hair length, although an auxin response was not required in these cells. Three-dimensional modelling of auxin flow in the root tip suggests that AUX1-dependent transport through non-hair cells maintains an auxin supply to developing hair cells as they increase in distance from the root tip, and sustains root-hair outgrowth. Experimental data support the hypothesis that instead of moving uniformly though the epidermal cell layer(3,12), auxin is mainly transported through canals that extend longitudinally into the tissue.
引用
收藏
页码:78 / U156
页数:19
相关论文
共 36 条
[1]   Intracellular trafficking and proteolysis of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism [J].
Abas, L ;
Benjamins, R ;
Malenica, N ;
Paciorek, T ;
Wirniewska, J ;
Moulinier-Anzola, JC ;
Sieberer, T ;
Friml, J ;
Luschnig, C .
NATURE CELL BIOLOGY, 2006, 8 (03) :249-256
[2]   Interactions of PIN and PGP auxin transport mechanisms [J].
Bandyopadhyay, A. ;
Blakeslee, J. J. ;
Lee, O. R. ;
Mravec, J. ;
Sauer, M. ;
Titapiwatanakun, B. ;
Makam, S. N. ;
Bouchard, R. ;
Geisler, M. ;
Martinoia, E. ;
Friml, J. ;
Peer, W. A. ;
Murphy, A. S. .
BIOCHEMICAL SOCIETY TRANSACTIONS, 2007, 35 :137-141
[3]   Positional information in root epidermis is defined during embryogenesis and acts in domains with strict boundaries [J].
Berger, F ;
Haseloff, J ;
Schiefelbein, J ;
Dolan, L .
CURRENT BIOLOGY, 1998, 8 (08) :421-430
[4]   The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots [J].
Blilou, I ;
Xu, J ;
Wildwater, M ;
Willemsen, V ;
Paponov, I ;
Friml, J ;
Heidstra, R ;
Aida, M ;
Palme, K ;
Scheres, B .
NATURE, 2005, 433 (7021) :39-44
[5]   Auxin transport promotes Arabidopsis lateral root initiation [J].
Casimiro, I ;
Marchant, A ;
Bhalerao, RP ;
Beeckman, T ;
Dhooge, S ;
Swarup, R ;
Graham, N ;
Inzé, D ;
Sandberg, G ;
Casero, PJ ;
Bennett, M .
PLANT CELL, 2001, 13 (04) :843-852
[6]   P-glycoprotein4 displays auxin efflux transporter-like action in Arabidopsis root hair cells and tobacco cells [J].
Cho, Misuk ;
Lee, Sang Ho ;
Cho, Hyung-Taeg .
PLANT CELL, 2007, 19 (12) :3930-3943
[7]   Cell identity mediates the response of Arabidopsis roots to abiotic stress [J].
Dinneny, Jose R. ;
Long, Terri A. ;
Wang, Jean Y. ;
Jung, Jee W. ;
Mace, Daniel ;
Pointer, Solomon ;
Barron, Christa ;
Brady, Siobhan M. ;
Schiefelbein, John ;
Benfey, Philip N. .
SCIENCE, 2008, 320 (5878) :942-945
[8]   Endocytosis, endosome trafficking, and the regulation of Drosophila development [J].
Fischer, Janice A. ;
Eun, Suk Ho ;
Doolan, Benjamin T. .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2006, 22 :181-206
[9]   Vectorial information for Arabidopsis planar polarity is mediated by combined AUX1, EIN2, and GNOM activity [J].
Fischer, Urs ;
Ikeda, Yoshihisa ;
Ljung, Karin ;
Serralbo, Olivier ;
Singh, Manoj ;
Heidstra, Renze ;
Palme, Klaus ;
Scheres, Ben ;
Grebe, Markus .
CURRENT BIOLOGY, 2006, 16 (21) :2143-2149
[10]   Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis [J].
Friml, J ;
Wisniewska, J ;
Benkova, E ;
Mendgen, K ;
Palme, K .
NATURE, 2002, 415 (6873) :806-809