Interactions of PIN and PGP auxin transport mechanisms

被引:86
作者
Bandyopadhyay, A.
Blakeslee, J. J.
Lee, O. R.
Mravec, J.
Sauer, M.
Titapiwatanakun, B.
Makam, S. N.
Bouchard, R.
Geisler, M.
Martinoia, E.
Friml, J.
Peer, W. A.
Murphy, A. S. [1 ]
机构
[1] Purdue Univ, Dept Hort, W Lafayette, IN 47907 USA
[2] Univ Tubingen, Ctr Plant Mol Biol, Tubingen, Germany
[3] Univ Zurich, Inst Plant Biol, Basel Zurich Plant Sci Ctr, CH-8007 Zurich, Switzerland
关键词
AUX1; auxin; detergent-resistant microdomain (DRM); pin-formed (PIN); P-glycoprotein (PGP); polar transport;
D O I
10.1042/BST0350137
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Polarized transport of the plant hormone auxin influences multiple growth processes in plants and is regulated by plasma-membrane-localized efflux and uptake carriers. The PGP (P-glycoprotein) ABC transporters (ATP-binding-cassette transporters), PIN (pin-formed) subfamily of major facilitator proteins and members of AUX/LAX families have been shown to independently transport auxin both in plonto and in heterologous systems. However, PIN- and PGP-mediated transport in hetefologous systems exhibits decreased substrate specificity and inhibitor-sensitivity compared with what is seen in plants and plant cells. To determine whether PIN-PGP interactions enhance transport specificity, we analysed interactions of the representative auxin-transporting PGPs with PIN1 and AUX1 in plonta and in heterologous systems. Here, we provide evidence that PINS and PGPs interact and function both independently and co-ordinately to control polar auxin transport and impart transport specificity and directionality. These interactions take place in protein complexes stabilized by PGPs in detergent-resistant microdomains.
引用
收藏
页码:137 / 141
页数:5
相关论文
共 36 条
[1]   A novel way to spread drug resistance in tumor cells: functional intercellular transfer of P-glycoprotein (ABCB1) [J].
Ambudkar, SV ;
Sauna, ZE ;
Gottesman, MM ;
Szakacs, G .
TRENDS IN PHARMACOLOGICAL SCIENCES, 2005, 26 (08) :385-387
[2]   Biochemical, cellular, and pharmacological aspects of the multidrug transporter [J].
Ambudkar, SV ;
Dey, S ;
Hrycyna, CA ;
Ramachandra, M ;
Pastan, I ;
Gottesman, MM .
ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1999, 39 :361-398
[3]   Reversible transport by the ATP-binding cassette multidrug export pump LmrA - ATP synthesis at the expense of downhill ethidium uptake [J].
Balakrishnan, L ;
Venter, H ;
Shilling, RA ;
van Veen, HW .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (12) :11273-11280
[4]   Regulating the regulator: the control of auxin transport [J].
Benjamins, R ;
Malenica, N ;
Luschnig, C .
BIOESSAYS, 2005, 27 (12) :1246-1255
[5]   Local, efflux-dependent auxin gradients as a common module for plant organ formation [J].
Benková, E ;
Michniewicz, M ;
Sauer, M ;
Teichmann, T ;
Seifertová, D ;
Jürgens, G ;
Friml, J .
CELL, 2003, 115 (05) :591-602
[6]  
Blakeslee JJ, 2006, PLANT CELL MONOGR, V1, P159, DOI 10.1007/7089_010
[7]   Auxin transport [J].
Blakeslee, JJ ;
Peer, WA ;
Murphy, AS .
CURRENT OPINION IN PLANT BIOLOGY, 2005, 8 (05) :494-500
[8]  
BLAKESLEE JJ, 2007, IN PRESS PLANT CELL
[9]   Plant gravitropism. Unraveling the ups and downs of a complex process [J].
Blancaflor, EB ;
Masson, PH .
PLANT PHYSIOLOGY, 2003, 133 (04) :1677-1690
[10]   Immunophilin-like TWISTED DWARF1 modulates auxin efflux activities of Arabidopsis P-glycoproteins [J].
Bouchard, Rodolphe ;
Bailly, Aurelien ;
Blakeslee, Joshua J. ;
Oehring, Sophie C. ;
Vincenzetti, Vincent ;
Lee, Ok Ran ;
Paponov, Ivan ;
Palme, Klaus ;
Mancuso, Stefano ;
Murphy, Angus S. ;
Schulz, Burkhard ;
Geisler, Markus .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (41) :30603-30612