Transcriptional regulation of a second flavodoxin gene from Klebsiella pneumoniae

被引：13

作者：

Achenbach, LA

Genova, EG

机构：

[1] Department of Microbiology, Southern Illinois University, Carbondale

来源：

GENE | 1997年 / 194卷 / 02期

关键词：

fldA; nifF; heat shock; fur protein; iron; sequence;

D O I：

10.1016/S0378-1119(97)00168-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A second flavodoxin gene, distinct from the nifF gene encoding nitrogenase flavodoxin, has been isolated from Klebsiella pneumoniae. This flavodoxin gene is a homologue of the E. coli fldA gene and is located 286 bp upstream of the K. pneumoniae fur gene. Primer extension analysis revealed an unusual promoter region upstream of the K. pneumoniae fldA gene that does not match the -35/-10 consensus sequence. Transcriptional analyses using a Fur titration assay and fldA gene fusions demonstrated that, unlike E. coli fldA, the K. pneumoniae fldA gene is not constitutively expressed. Rather, the fldA gene of K. pneumoniae is repressed in high iron conditions by the ferric uptake regulator (Fur) protein. Expression of K. pneumoniae fldA is also induced by heat shock but not by salt stress. (C) 1997 Elsevier Science B.V.

引用

收藏

页码：235 / 240

页数：6

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