Sterilization effect of atmospheric plasma on Escherichia coli and Bacillus subtilis endospores

被引：91

作者：

Hong, Y. F. ^{[1
]}

Kang, J. G. ^{[1
]}

Lee, H. Y. ^{[1
]}

Uhm, H. S. ^{[1
]}

Moon, E. ^{[2
]}

Park, Y. H. ^{[1
]}

机构：

[1] Ajou Univ, Dept Mol Sci & Technol, Suwon 443749, South Korea

[2] Ajou Univ, Dept Biol, Suwon 443749, South Korea

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2009年 / 48卷 / 01期

关键词：

Bacillus subtilis spores; Escherichia coli; low-temperature atmospheric plasma; oxygen radicals; plasma sterilization;

D O I：

10.1111/j.1472-765X.2008.02480.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Escherichia coli and Bacillus subtilis spores were treated with an atmospheric plasma mixture created by the ionization of helium and oxygen to investigate the inactivation efficiency of a low-temperature plasma below 70 degrees C. An electrical discharge plasma was produced at a radio frequency (RF) of 13.56 MHz, connected to a perforated circular electrode with a discharge spacing of 1-15 mm. The discharge gas was helium with 0-2% oxygen. For the plasma treatment, a dried E. coli cell or B. subtilis endospore suspension on a cover-glass was exposed to oxygen downstream of the plasma from holes in an RF-powered electrode. The sterilization effect of the RF plasma was highest with 0.2% oxygen, corresponding to the maximum production of oxygen radicals. Oxygen radicals generated by RF plasma are effective for the destruction of bacterial cells and endospores. Low-temperature atmospheric plasma can be used for the disinfection of diverse objects, especially for the inactivation of bacterial endospores.

引用

页码：33 / 37

页数：5

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