Pyrolysis-gas chromatography-mass spectrometry of synthetic neuromelanins

被引：18

作者：

Dzierzega-Lecznar, A ^{[1
]}

Chodurek, E ^{[1
]}

Stepien, K ^{[1
]}

Wilczok, T ^{[1
]}

机构：

[1] Med Univ Silesia, Dept Mol Biol Biochem & Biopharm, PL-41200 Sosnowiec, Poland

来源：

JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS | 2002年 / 62卷 / 02期

关键词：

pyrolysis-gas chromatography-mass spectrometry; dopamine; cysteinyldopamine; neuromelanin;

D O I：

10.1016/S0165-2370(01)00122-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Synthetic neuromelanins obtained from dopamine and/or 5-S-cysteinyldopamine were pyrolyzed at 770 degreesC. The thermal degradation products were separated by gas chromatography and identified by mass spectrometry. Pyrolytic patterns of melanins containing 5-S-cysteinyldopamine-derived component were different from that of dopamine-melanin. Major pyrolytic products of 5-S-cysteinyldopamine-originated units were indentified as 2H-1,4-benzothiazin-5-one, 4-hydroxybenzothiazole and thiazoloisoquinoline and their alkyl derivatives. The results obtained indicate that pyrolysis-gas chromatography-mass spectrometry method is suitable for identification of 5-S-cysteinyldopamine-derived units in synthetic melanin copolymers and it could be applied for structural analysis of natural neuromelanin. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：239 / 248

页数：10

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