Emended description of the genus Phyllobacterium and description of four novel species associated with plant roots:: Phyllobacterium bourgognense sp nov., Phyllobacterium ifriqiyense sp nov., Phyllobacterium leguminum sp nov and Phyllobacterium brassicacearum sp nov.

被引:91
作者
Mantelin, S
Fischer-Le Saux, M
Zakhia, F
Béna, G
Bonneau, S
Jeder, H
de Lajudie, P
Cleyet-Marel, JC
机构
[1] LSTM, UMR 113, F-34398 Montpellier 05, France
[2] INRA, UMR 77, F-49071 Beaucouze, France
[3] Inst Reg Arides, Nahal Gabes, Tunisia
关键词
D O I
10.1099/ijs.0.63911-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Gram-negative bacteria were isolated from the rhizoplane of Brassica napus in France and from root nodules of Argyrolobium, uniflorum, Astragalus algerianus and Lathyrus numidicus growing in the infra-arid zone of southern Tunisia. Based on phylogenetic analysis of the 16S rRNA gene sequences, the seven isolates belong to the Alphaproteobacteria and are related to Phyllobacterium myrsinacearum strains. The isolates formed three clusters; clusters A and C consist of Tunisian strains, whereas cluster B consists of two strains from Brassica napus from France. Phylogenetic reconstruction based on the atpD gene strongly supports their affiliation to the genus Phyllobacterium. DNA-DNA hybridizations revealed that (i) none of the isolates belong to the species P. myrsinacearum, (ii) clusters A and C represent two distinct genomospecies and (iii) the two strains of cluster B represent two separate genomospecies. Distinctive phenotypic features were deduced from numerical analysis of phenotypic data. Based on this polyphasic approach, four novel species are proposed: Phyllobacterium leguminum sp. nov. (type strain ORS 1419(T) = CFBP 6745(T) = LMG 22833(T)), Phyllobacterium ifriqiyense sp. nov. (type strain STM 370(T) = CFBP 6742(T) = LMG 22831(T)), Phyllobacterium brassicacearum sp. nov. (type strain STM 196(T) = CFBP 5551(T) = LMG 22836(T)) and Phyllobacterium bourgognense sp. nov. (type strain STM 20(T) = CFBP 5553(T) =LMG 22837(T)). The description of the genus Phyllobacterium is emended accordingly.
引用
收藏
页码:827 / 839
页数:13
相关论文
共 60 条
[31]   DETERMINATION OF BASE COMPOSITION OF DEOXYRIBONUCLEIC ACID FROM ITS THERMAL DENATURATION TEMPERATURE [J].
MARMUR, J ;
DOTY, P .
JOURNAL OF MOLECULAR BIOLOGY, 1962, 5 (01) :109-&
[32]  
Martínez J, 2004, INT MICROBIOL, V7, P261
[33]   SURVEY OF INDIGENOUS BACTERIAL ENDOPHYTES FROM COTTON AND SWEET CORN [J].
MCINROY, JA ;
KLOEPPER, JW .
PLANT AND SOIL, 1995, 173 (02) :337-342
[34]  
Mergaert J, 2002, INT J SYST EVOL MICR, V52, P1821, DOI 10.1099/00207713-52-5-1821
[35]   Identity and potential functions of heterotrophic bacterial isolates from a continuous-upflow fixed-bed reactor for denitrification of drinking water with bacterial polyester as source of carbon and electron donor [J].
Mergaert, J ;
Boley, A ;
Cnockaert, MC ;
Müller, WR ;
Swings, J .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 2001, 24 (02) :303-310
[36]   GENOTYPIC AND PHENOTYPIC DIVERSITY OF RHIZOBIUM ISOLATED FROM CHICKPEA (CICER-ARIETINUM L) [J].
NOUR, SM ;
CLEYETMAREL, JC ;
BECK, D ;
EFFOSSE, A ;
FERNANDEZ, MP .
CANADIAN JOURNAL OF MICROBIOLOGY, 1994, 40 (05) :345-354
[37]   Engineering root exudation of lotus toward the production of two novel carbon compounds leads to the selection of distinct microbial populations in the rhizosphere [J].
Oger, PM ;
Mansouri, H ;
Nesme, X ;
Dessaux, Y .
MICROBIAL ECOLOGY, 2004, 47 (01) :96-103
[38]   THERMAL-DENATURATION OF PARTLY PURIFIED BACTERIAL DEOXYRIBONUCLEIC-ACID AND ITS TAXONOMIC APPLICATIONS [J].
OWEN, RJ ;
LAPAGE, SP .
JOURNAL OF APPLIED BACTERIOLOGY, 1976, 41 (02) :335-340
[39]   RAPID EXTRACTION OF BACTERIAL GENOMIC DNA WITH GUANIDIUM THIOCYANATE [J].
PITCHER, DG ;
SAUNDERS, NA ;
OWEN, RJ .
LETTERS IN APPLIED MICROBIOLOGY, 1989, 8 (04) :151-156
[40]   MODELTEST: testing the model of DNA substitution [J].
Posada, D ;
Crandall, KA .
BIOINFORMATICS, 1998, 14 (09) :817-818