The promotion of secondary structures in single-stranded DNA by drugs that bind to duplex DNA: an atomic force microscopy study

被引：33

作者：

Adamcik, Jozef ^{[1
]}

Valle, Francesco ^{[2
]}

Witz, Guillaume ^{[1
]}

Rechendorff, Kristian ^{[1
]}

Dietler, Giovanni ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Phys Mat Vivante, CH-1015 Lausanne, Switzerland

[2] ISMN, CNR, Sez Bologna, I-40129 Bologna, Italy

来源：

NANOTECHNOLOGY | 2008年 / 19卷 / 38期

关键词：

D O I：

10.1088/0957-4484/19/38/384016

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 [材料科学与工程]; 080502 [材料学];

摘要：

We study the behavior of single-stranded DNA (ssDNA) in the presence of well-known drugs with either an intercalating binding mode, such as daunorubicin, actinomycin D, and chloroquine, or a minor groove binding mode, such as netropsin and berenil, by atomic force microscopy (AFM). At very low salt conditions, ssDNA molecules adopt an unstructured conformation without secondary structures. We observe that under these conditions additions of drugs that bind to double-stranded DNA ( dsDNA) promote the formation of secondary structures in ssDNA. Furthermore, with an increase of concentration of the drugs, the extension as well as the thermal stabilization of these hairpins was observed.

引用

页数：7

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