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Morphological alterations and DNA fragmentation in oocytes from primordial and primary follicles after freezing-thawing of ovarian cortex in sheep
被引:54
作者:
Demirci, B
[1
]
Salle, B
Frappart, L
Franck, M
Guerin, JF
Lornage, J
机构:
[1] Ecole Natl Vet Lyon, Lab Zootech, F-69280 Marcy Letoile, France
[2] Hop Edouard Herriot, CECOS, Dept Med & Biol Reprod, Lyon, France
[3] Hop Edouard Herriot, Lab Anat Pathol, Lyon, France
关键词:
cryopreservation;
ovarian tissue;
sheep;
DNA fragmentation;
D O I:
10.1016/S0015-0282(01)03205-8
中图分类号:
R71 [妇产科学];
学科分类号:
100211 ;
摘要:
Objective: To evaluate DNA fragmentation in the oocyte of primordial and primary follicles and morphology of these follicles after freezing and thawing of ovarian cortex in sheep using two freezing protocols. Design: Fragmentation of DNA was evaluated by the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) technique. Setting: Fertility clinic in a large university hospital. Animals: Five- to 6-month-old lambs. Intervention(s): Two-millimeter-thick slices of hemi-ovary cortex were prepared. Main Outcome Measure(s): Histological structure and DNA fragmentation. Result(s): In the frozen fragments, the percentage of morphologically normal follicles was significantly lower for both protocols compared with the case of the control group of fresh fragments. There was no significant difference between the two types of freezing protocols (60.4% +/- 13.2% vs. 68.4% +/- 13.7%). However, the distribution of abnormalities (nucleus, cytoplasm, and nucleus and cytoplasm) was dissimilar. The results of the TUNEL technique for the three groups showed no significant difference, but the percentage of the TUNEL-positive follicles was slightly lower for the frozen fragments for both protocols with respect to the control group. Conclusion(s): The freezing and thawing process of the ovarian cortex does not induce fragmentation of the DNA on the oocyte of primary and primordial follicles. (C) 2002 by American Society for Reproductive Medicine.
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页码:595 / 600
页数:6
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