An MCP-based system for beta autoradiography

被引：23

作者：

Lees, JE ^{[1
]}

Pearson, JF

Fraser, GW

Hales, JM

Richards, PG

机构：

[1] Univ Leicester, Dept Phys & Astron, Space Res Ctr, Leicester LE1 7RH, Leics, England

[2] Univ Leicester, Dept Med & Therapeut, Div Dermatol, Leicester LE1 7RH, Leics, England

[3] MRC, Toxicol Unit, Leicester LE1 9HN, Leics, England

来源：

IEEE TRANSACTIONS ON NUCLEAR SCIENCE | 1999年 / 46卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1109/23.775591

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We describe a pre-commercial prototype autoradiography system which can be used to image a variety of radiolabelled biological samples, ranging from lymphocyte proliferation assays to semi-thin whole body tissue slices. These samples can be imaged with high spatial resolution (similar to 70 microns) and high sensitivity (eg. 3H - minimum detectable activity 0.001Bq in 20 hours) over a wide (5-6 orders of magnitude) dynamic range. Specimens are automatically presented, through a vacuum load-lock, to an imaging microchannel plate (MCP) detector operating in high (similar to 10(-6)mbar) vacuum. We present images obtained from tritium labelled samples and discuss the importance of the new system for a variety of biological techniques.

引用

页码：636 / 638

页数：3

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